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首页> 外文会议>Annual Meeting of The National Mastitis Council (U.S.). >A New Method for Obtaining Biopsies from the Teat Canal of Dairy Cows
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A New Method for Obtaining Biopsies from the Teat Canal of Dairy Cows

机译：从乳制奶牛奶头运河中获取活检的一种新方法

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摘要

The teat canal is the cylindrical duct at the lower end of the teat, lined by a stratified cornified squamous epithelium. This cornified keratin lining is an important innate defence mechanism against mastitis, as it acts as a physical and chemical protective barrier. Its thickness and other characteristics of the teat canal have been related to the susceptibility of cows to mastitis (Hillerton and Lacy-Hulbert 1995; Davidov et al. 2011). A technique used to evaluate characteristics of the keratin layer was to extract loose desquamating keratin from the teat canal. However, as keratin is a layer of a cornified epithelium, the amount of desquamated keratin is unlikely to represent the total amount of keratin present in the canal. For histological examination of the teat canal, cows were sacrificed. New sampling techniques are needed to better understand the role of keratin in the teat canal in protecting cows' mammary glands from mastitis. The studies presented here aimed to develop a biopsy technique to obtain teat canal epithelium from live cows.
机译：奶头运河是乳头下端的圆柱形管道，由分层的鳞状上皮内衬。这种角质角蛋白衬里是一种重要的天生防御机制，免受乳腺炎，因为它充当了物理和化学防护障碍。它的厚度和奶头运河的其他特征与奶牛到乳腺炎的易感性有关（Hillerton和Lacy-Hulbert 1995; Davidov等，2011）。用于评估角蛋白层的特征的技术是从乳头运河中提取松散的脱皮角蛋白。然而，随着角蛋白是一种颅骨上皮层，脱皮角蛋白的量不太可能代表运河中存在的角蛋白的总量。对于乳头运河的组织学检查，处死奶牛。需要进行新的抽样技术，以更好地了解角蛋白在乳腺炎中保护乳腺炎的作用中的角色。这里提出的研究旨在开发活检技术，以获得来自活奶牛的乳酸管上皮。

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