Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles

机译：链霉抗生物素蛋白在酶活性生物素化细菌磁性颗粒的遗址选择性固定

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Biotinylated magnetic nanoparticles were constructed by displaying biotin acceptor peptide (BAP) on the surfaces of bacterial magnetic particles (BacMPs) synthesized by Magneto spirillum magneticum AMB-1. Both BAP and green fluorescent protein (GFP) were fused to Mms13 that was isolated from BacMP membranes. The localization of the fusion protein, BAP-Mms13-GFP, was confirmed by fluorescence analysis. BacMPs that expressed BAP-Mms13-GFP (BAP/GFP-BacMPs) were extracted from bacterial cells and incubated with biotin and Escherichia coli biotin ligase. The in vitro biotinylation of BAP/GFP-BacMPs was confirmed using alkaline phosphatase (ALP)-labeled streptavidin. The conjugation system developed in this study provides a method for producing biotin- or streptavidin-labeled magnetic nanoparticles without the use of a crosslinker reagent. Various functional materials can be immobilized site-selectively onto these uniquely designed BacMPs. By combining this site-selective biotinylation technology and protein display methodology, increasingly innovative and attractive magnetic nano-materials can be constructed.

机译：通过在由磁螺藻渣-11合成的细菌磁性颗粒（BACMP）的表面上显示生物素受体肽（BAP）来构建生物素化的磁性纳米颗粒。将BAP和绿色荧光蛋白（GFP）融合到均来自BACMP膜中分离的MMS13。通过荧光分析证实了融合蛋白，BAP-MMS13-GFP的定位。从细菌细胞中提取表达BAP-MMS13-GFP（BAP / GFP-BACMP）的BACMP，并与生物素和大肠杆菌生物素连接酶孵育。使用碱性磷酸酶（ALP） - 标记的链霉抗生物素蛋白证实了BAP / GFP-BACMP的体外生物素化。本研究开发的共轭系统提供了一种用于在不使用交联剂试剂的情况下生产生物素或链霉蛋白标记的磁性纳米颗粒的方法。各种功能材料可以选择性地固定在这些独特设计的Bacmps上。通过组合该站点选择性生物素化技术和蛋白质显示方法，可以构建越来越创新的创新和有吸引力的磁性纳米材料。

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《Symposium on From Biological Materials to Biomimetic Material Synthesis》|2008年||共6页
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Yoshiaki Maeda; Tomoko Yoshino; Haruko Takeyama; Masaaki Takahashi; Harami Ginya; Junko Asahina; Hideji Tajima; Tadashi Matsunaga;
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1. Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles [J] . Yoshiaki Maeda, Tomoko Yoshino, Masaaki Takahashi, Applied and Environmental Microbiology . 2008,第16期

机译：链霉亲和素在体内和体内生物素化细菌磁性颗粒上的非共价固定
2. Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles [J] . Yoshiaki Maeda, Tomoko Yoshino, Masaaki Takahashi, Applied and Environmental Microbiology . 2008,第16期

机译：链霉亲和素在体内和体内生物素化细菌磁性颗粒上的非共价固定
3. Magnetic particles as labels in bioassays: Interactions between a biotinylated gold substrate and streptavidin magnetic particles [J] . De Palma R, Liu CX, Barbagini F, The journal of physical chemistry, C. Nanomaterials and interfaces . 2007,第33期

机译：磁性颗粒作为生物测定中的标记：生物素化的金底物和链霉亲和素磁性颗粒之间的相互作用
4. Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles [C] . Yoshiaki Maeda, Tomoko Yoshino, Haruko Takeyama, MRS spring meeting symposium . 2008

机译：链霉亲和素在酶促生物素化细菌磁性颗粒上的定点固定
5. Investigating cell adhesion to controlled surface chemistry via self-assembly of binary composition alkylthiol monolayers, streptavidin immobilization, and cell receptor ligand attachment. [D] . Nelson, Kjell Erik. 2003

机译：通过二元组合烷硫醇单分子层的自组装，链霉亲和素固定和细胞受体配体附着，研究细胞对受控表面化学的粘附。
6. Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles [O] . Yoshiaki Maeda, Tomoko Yoshino, Masaaki Takahashi, 2008

机译：链霉亲和素在体内和体内生物素化细菌磁性颗粒上的非共价固定
7. Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles▿ [O] . Maeda, Yoshiaki, Yoshino, Tomoko, Takahashi, Masaaki, 2008

机译：链霉亲和素在体内和体内生物素化细菌磁性粒子上的非共价固定
8. Intelligent Biomaterials Based on Langmuir-Blodgett Monolayer Films: BiotinylatedPolymers-Streptavidin and Biotinylated Lipid-Streptavidin Recognition Incorporating Photodynamic Proteins [R] . Marx, K. A., Samuelson, L. A., Kamath, M., 1994

机译：基于Langmuir-Blodgett单层膜的智能生物材料：生物素化聚合物 - 链霉抗生物素蛋白和生物素化脂质 - 链霉抗生物素蛋白识别结合光动力蛋白

Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles

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