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首页> 外文会议>International Conference on Mushroom Biology and Mushroom Products >Biosynthesis of Immunostimulatory Compounds from Ganoderma sp. from European Habitats by Submerged and Solid State Cultivation
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Biosynthesis of Immunostimulatory Compounds from Ganoderma sp. from European Habitats by Submerged and Solid State Cultivation

机译：来自Ganoderma SP的免疫刺激化合物的生物合成。 来自淹没和固态培养的欧洲栖息地

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An original strain of Ganoderma lucidum MZKI G97, was isolated from Slovenian forests was cultivated in a liquid substrate based on potato dextrose and olive oil in a 10L laboratory stirred tank bioreactor as well as in 15L solid state cultivation in a horizontal reactor in substrate based on beech sawdust and mineral media. In submerged cultivation, up to 17.0 gL~(-1) of dry fungal biomass was obtained in a fed batch process. From the mycelium biomass, 1.7 gL~(-1) of extracellular and 0.45 gL~(-1) of intracellular polysaccharides were isolated, which were mainly β-D-glucans. Polysaccharide fractions, tested in vitro for the induction of cytokine synthesis in primary cultures of human mononuclear cells from a buffy coat of healthy donors, induced 3.0-630 pg mL~(-1) of TNF-α, and 1.23-2.18 pg mL~(-1) of IFN-γ which is comparable to the activity of romurtide, a supporting drug in cancer therapy.
机译：在基于马铃薯葡萄球菌和橄榄油的液体基质中，在10L实验室搅拌釜生物反应器中培养了Ganoderma lucidumMzki G97的原始菌株，并在基于底物的水平反应器中的15L固态培养中，在液体基质中培养。 山毛榉木屑和矿物媒体。 在淹没的栽培中，在美联储的分批过程中获得高达17.0gl〜（-1）干真菌生物量。 从菌丝体生物质，分离出细胞内多糖的细胞外和0.45gl〜（-1）的1.7gl〜（-1），主要是β-d-葡聚糖。 多糖级分，在体外测试用于诱导来自一种来自健康供体的人单核细胞的原代培养物中细胞因子合成，诱导3.0-630pg ml〜（-1）的TNF-α，1.23-2.18pg ml〜 （-1）IFN-γ的IFN-γ相当，癌症治疗中的支持药物的活性。

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