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首页> 外文会议>International Conference on Mushroom Biology and Mushroom Products >Gene Expression of a New Metalloprotease from Pleurotus ostreatus is Developmentally Regulated
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Gene Expression of a New Metalloprotease from Pleurotus ostreatus is Developmentally Regulated

机译：从Pleurotus ostreatus的新金属蛋白酶的基因表达是发育调节的

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A metzincin family metalloprotease cDNA (PoMTP) was cloned from Pleurotus ostreatus. Full-length cDNA sequence (1,140bp) of PoMTP contained an open reading frame (ORF) of 870bp encoding a protein product of 290 amino acids. The deduced amino-acid sequences of PoMTP contained an extensive zinc-binding consensus sequence and a so-called Met-turn sequence that are typical for the metzincin family of metalloproteases. Four cysteine residues were also observed in the zinc-binding region of PoMTP amino-acid sequence, which are known to be important for the structure and the function of some subfamilies of the metzincins. Searches of the GenBank protein database showed that the amino-acid sequence of PoMTP over the whole amino-acid sequence has a high identity with metalloproteases or hypothetical proteins from one basidiomycete of Ustilago maydis, and four ascomycetes of Magnaporthe, Metarhizium anisopliae var. anisopliae, Aspergillus nidulans and Neurospora. Although no information about the function of these proteins is known at present, all these proteins have an extended zinc-binding sequence and the Met turn sequence. Furthermore, the putative metzincin PoMTP metalloprtease indicated no significant homology with any other pre-reported mushroom metalloproteases, which are fibrinolytic metalloprotease, mitochondrial intermediate peptidase and mitochondrial processing peptidase. Real-time PCR, quantitative RT-PCR and Northern blot analyses indicated the PoMTP mRNA to be abundant at primordial and fruit body stages, but scarce at the mycelial stage, suggesting that the PoMTP metalloprotease plays an important role in mushroom fruiting.
机译：从Pleurotus Ostreatus克隆了甲磺酸族家族金属蛋白酶cDNA（POMTP）。 POMTP的全长cDNA序列（1,140bp）含有870bp的开放阅读框（ORF），编码290个氨基酸的蛋白质产物。 POMTP的推导氨基酸序列含有广泛的锌结合共识序列和所谓的MET-转序列，其典型的金属蛋白族的金属蛋白酶。在POMTP氨基酸序列的锌结合区域中也观察到四个半胱氨酸残基，已知是对茂硫簇的一些亚壳的结构和功能是重要的。 Genbank蛋白质数据库的搜索表明，整个氨基酸序列上的POMTP的氨基酸序列具有高度高度的ustilago maydis的一种底碱基的金属蛋白酶或假想蛋白质，以及Magnaporthe的四个ascarhizium anisopliae var。 Anisopliae，Aspergillus nidulans和neurospora。尽管目前没有已知有关这些蛋白质的功能的信息，但所有这些蛋白质都具有延伸的锌结合序列和满足匝数。此外，推定的富硒素POMTP金属抗妥妥酶表明，与任何其他预报告的蘑菇金属蛋白酶没有显着同源性，其是纤维蛋白溶解金属蛋白酶，线粒体中间肽酶和线粒体加工肽酶。实时PCR，定量RT-PCR和Northern印迹分析表明POMTP mRNA在原始和果实阶段浓郁，但在菌丝体阶段稀缺，表明POMTP金属蛋白酶在蘑菇果实中起着重要作用。

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