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首页> 外文会议>International Conference on Mushroom Biology and Mushroom Products >Determination of Pseudomonas tolaasii Threshold Concentrations Required to Produce Symptoms of Bacterial Blotch Disease in Pleurotus eryngii
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Determination of Pseudomonas tolaasii Threshold Concentrations Required to Produce Symptoms of Bacterial Blotch Disease in Pleurotus eryngii

机译：在Pleurotus erryngii中产生产生细菌斑疾病症状所需的甲状腺肿甲状腺素阈值浓度的测定

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Pseudomonas tolaasii causes bacterial blotch disease of many cultivated fungi including Agaricus bisporus and Pleurotus spp. Concentration and amount of bacterial inoculum are critical for symptom development. A minimal concentration of 2 x 10~6 cells per inoculation site has been reported for pilei surfaces of A. bisporus. However, the same concentration of bacteria was not sufficient to produce symptoms on basidiomata of P. eryngii. To determine the effect of inoculum concentration on disease development, we produced P. eryngii (WC-888) on a mixture (contained in 1050 ml polypropylene bottles) of cottonseed hulls (62%), ground soybean (6%), oak sawdust (27%), corn distiller's waste (4%), and calcium sulphate (1%). Mushrooms were harvested at the same developmental stage (margins and pileus completely flat) for disease assays. Pseudomonas tolaasii was grown for 40 hours in King's B broth. The bacterial suspension (20 ml) was centrifuged (6,500 x g) and rinsed twice with sterile distilled water. From this stock concentration, several bacterial suspensions, 0.1 - 2.0 OD_(450), were prepared. The external layer of the pilei and freshly cut pilei tissue of the P. eryngii basidiomata were inoculated (50 μl). Mushroom pilei were incubated in individual moist chambers at 16°C for 48 hours. Presence or absence of disease symptoms was recorded. A minimal concentration of 5 x 10~6 bacterial cells (0.3 OD_(450)) per inoculation site was necessary for symptom expression on surfaces of cut pileus tissue. However, a nearly 5-fold higher concentration of bacteria [2.6 x 10~7 (1.6 OD_(450))] was required to produce symptoms on the intact surface of the pileus. Bacterial concentration and tissue type were critical factors in disease development and symptom expression.
机译：Pseudomonasolaasii导致许多栽培真菌的细菌污染疾病，包括姬松动物和Pleurotus spp。细菌接种物的浓度和量对于症状发展至关重要。针对A. Bisporus的Pili表面报道了每种接种位点的最小浓度为2×10〜6个细胞。然而，相同的细菌浓度不足以在P.Eryngii的碱基上产生症状。为了确定接种物浓度对疾病发展的影响，我们在棉籽壳（62％），煤籽（6％），橡木锯末（6％），橡木（6％），橡木锯末（6％）生产的混合物（含有1050ml聚丙烯瓶中）的菌状物（WC-888）。 27％），玉米蒸馏器的废物（4％）和硫酸钙（1％）。在同样的发育阶段（边缘和菌落完全平坦）收获蘑菇，用于疾病测定。在国王的B肉汤中，Pseudomonas tolaasii在王后长40个小时。将细菌悬浮液（20mL）离心（6,500×G）并用无菌蒸馏水冲洗两次。从该股票浓度，制备了几种细菌悬浮液，0.1-2.0 OD_（450）。 PILI的外部层和新切割P.ERYNGII碱基瘤的李组织接种（50μL）。将蘑菇盒在16℃下在各个湿室中孵育48小时。记录疾病症状的存在或不存在。症状表达在切割菌膜表面的症状表达需要每种接种位点的最小浓度为5×10〜6个细菌细胞（0.3 OD_（450））。然而，需要较高5倍的细菌浓度[2.6×10〜7（1.60D_（450））]在脐带的完整表面上产生症状。细菌浓度和组织类型是疾病发展和症状表达的关键因素。

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