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首页> 外文会议>International Conference on Mushroom Biology and Mushroom Products >Gene Transfer In Edible Mushrooms
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Gene Transfer In Edible Mushrooms

机译：在食用蘑菇中的基因转移

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摘要

Distinct methods of gene transfer in fungi are protoplast fusion, uptake of foreign genetic materials and transformation. The protoplast fusion products between compatible strains, Pleurotus ostreatus and P. florida, formed heterokaryons,while fusants between incompatible strains such as P. cornucopiae +P. florida, P. ostreatus + Ganoderma applanatum, P. florida + Ganoderma lucidum, and P. ostreatus + Flammulina velutipes formed synkaryons that retained genes from both parents. Transfer of the isolated nuclei from Lentinula edodes into protoplasts of P. florida was induced. Restriction enzyme-mediated integration was used to transform uracil auxotrophs of P. ostreatus to prototrophy. Two of nine uracil auxotrophs obtained were transformed to prototrophy using plasmid pTRura 3-2 that contains the orotidine monophosphate decarboxylase (ura3) gene from Trichoderma reesei. This review will discuss these aspects.
机译：真菌中基因转移的不同方法是原生质体融合，对外遗传物质的吸收和转化。 兼容菌株，Pleurotus Ostreatus和P.佛罗里达州的原生质体融合产物，形成的异质核龙，而P. Cornucopiae + P等菌株之间的定义。 佛罗里达州，P. Ostreatus + Ganoderma Applanatum，P.Florida + Ganoderma lucidum，以及P. Ostreatus + Flammulina Velutipes形成了从父母两种父母保留基因的骨颈。 将分离的核从甘氨酸核心转移到佛罗里达州P.佛罗里达州的原生质体中。 限制酶 - 介导的整合用于将P. Ostreatus的尿嘧啶助剂转化为肌醇萎缩。 使用质粒ptrura 3-2将获得的九个尿嘧啶助流膜中的两种转化为原发生冰，其含有来自Trichoderma Reesei的ortidine单磷酸脱羧酶（URA3）基因。 此评论将讨论这些方面。

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