Over-expression of a NAD-Malic Enzyme Gene from Rice in Arabidopsis thaliana Confers Tolerances to Several Abiotic Stresses

机译：拟南芥中稻米的NAD-苹果酶基因的过度表达将耐受性对几种非生物胁迫赋予耐受性

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In order to investigate the role of a rice NAD-malic enzyme gene (OsNAD-ME1) under different abiotic stresses, OsNAD-ME1 was constructed into plant expression vector and transformed into Agrobacterium for infecting Arabidopsis thaliana. There were higher transcriptional levels of OsNAD-ME1 in homozygous transgenic lines compared with WT plants as well as higher NAD-ME activity. WT and transgenic Arabidopsis were treated with NaCl, NaHCO_3, mannitol and H2O2. And then their root lengths, crown widths and fresh weights were measured and compared. The results showed that over-expression of OsNAD-ME1 in transgenic Arabidopsis increased the resistance to abiotic stresses, which indicated that OsNAD-ME1 was related to stress tolerance.

机译：为了探讨水稻NAD-meric酶基因（OSNAD-ME1）在不同的非生物胁迫下的作用，奥斯奈-ME1构建成植物表达载体并转化到土壤杆菌中，用于感染拟南芥。与WT植物以及较高的NAD-ME活性相比，纯合转基因系中奥纳德-ME1的转录水平较高。用NaCl，NaHCO_3，甘露醇和H 2 O 2处理WT和转基因拟南芥。然后测量并比较它们的根长度，冠状宽度和新鲜重量。结果表明，转基因拟南芥中的奥斯奈-ME1的过表达增加了对非生物胁迫的抗性，表明奥斯奈-ME1与应力耐受性有关。

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《CBCME 2011;International Conference on Biotechnology, Chemical and Materials Engineering》|2012年||共4页
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作者
Hao Zhou; Shenkui Liu; Chuanping Yang;
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中图分类 71.22083;
关键词
Malic enzyme gene; Oryza sativa L.; Transgenic Arabidopsis; Abiotic stress tolerances;

机译：苹果酶基因;Oryza sativa L.;转基因拟南芥;非生物胁迫耐受性;

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1. Expression of an NADP-malic enzyme gene in rice (Oryza sativa. L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance [J] . Shenkui Liu, Yuxiang Cheng, Xinxin Zhang, Plant Molecular Biology . 2007,第1a2期

机译：NADP-苹果酸酶基因在水稻（Oryza sativa。L）中的表达受环境胁迫的诱导。拟南芥中基因的过表达赋予盐和渗透胁迫耐受性
2. Expression of an NADP-malic enzyme gene in rice (Oryza sativa. L) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance [J] . Liu SK, Cheng YX, Zhang XX, Plant Molecular Biology . 2007,第1a2期

机译：NADP-苹果酸酶基因在水稻（Oryza sativa。L）中的表达受环境胁迫的诱导。拟南芥中基因的过表达赋予盐和渗透胁迫耐受性
3. Over-expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress. [J] . Kotchoni S O, Kuhns C, Ditzer A, Plant, Cell & Environment . 2006,第6期

机译：拟南芥中不同醛脱氢酶基因的过量表达赋予对非生物胁迫的耐受性，并保护植物免受脂质过氧化和氧化胁迫。
4. Over-expression of a NAD-Malic Enzyme Gene from Rice in Arabidopsis thaliana Confers Tolerances to Several Abiotic Stresses [C] . Hao Zhou, Shenkui Liu, Chuanping Yang International conference on biotechnology, chemical and materials engineering . 2012

机译：拟南芥中水稻NAD-苹果酶基因的过表达赋予对几种非生物胁迫的耐受性
5. Genome evolution and the genetics of abiotic stress tolerance in Arabidopsis thaliana and Arabidopsis lyrata. [D] . DeRose-Wilson, Leah J. 2010

机译：拟南芥和拟南芥的基因组进化及非生物胁迫耐受性的遗传学。
6. MhYTP1 and MhYTP2 from Apple Confer Tolerance to Multiple Abiotic Stresses in Arabidopsis thaliana [O] . Na Wang, Tianli Guo, Ping Wang, -1

机译：苹果的MhYTP1和MhYTP2赋予了拟南芥多种非生物胁迫耐受性
7. Over-expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress [O] . SIMEON O. KOTCHONI, CHRISTINE KUHNS, ANDREA DITZER, 2006

机译：拟南芥的不同醛脱氢酶基因的过表达赋予非生物胁迫的耐受性，保护植物免受脂质过氧化和氧化应激

Over-expression of a NAD-Malic Enzyme Gene from Rice in Arabidopsis thaliana Confers Tolerances to Several Abiotic Stresses

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