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首页> 外文会议>Asian Pacific Confederation of Chemical Engineering congress >An integrated extraction system for enhancing rhamnolipid productivity by Pseudomonas aeruginosa ZJU
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An integrated extraction system for enhancing rhamnolipid productivity by Pseudomonas aeruginosa ZJU

机译：一种综合的提取系统，用于增强假单胞菌Aeruginosa ZJU的菌毒素生产率

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It has been well known that rhamnolipids as potential natural biosurfactants with higher biodegradability, lower toxicity and irritancy have a wide range of potential applications in enhancing oil recovery, biodegradation,bioremediation and soil washing. However, mass production of rhamnolipid on industrial scale was largely inhibited by foaming. In fermentation of biosurfactant,rapid agitation and large aeration usually generate a bundle of foam even at an early stage and such foam generation was a severe problem for the subsequent fermentation due to contamination, instability and low efficiency. In this paper, Pseudomonas aeruginosa ZJU was fermented for producing rhamnolipids by using waste frying oils as the sole carbon source. To improve the efficiency of rhamnolipid production, a continuous integrated system of foam extraction together with recovery of nutrition and microbials was introduced into the fermentation apparatus. In this continuous integrated system, foams were allowed to flow out of the bioreactor through an external loop into a collection vessel for extracting rhamnolipids by organic solvents.The aqueous solution enriched with medium compositions and microbials were pumped back to the fermentor. Ethyl acetate and ethanol at various volume and extraction time were individually evaluated for such use. It was found that the foam extraction by ethanol greatly enhanced the productivity of rhamnolipids to 50 g/L.Besides, downstream processing of rhamnolipids was more easily handled when ethanol served as extracting agent. In summary, ethanol as low cost, less toxic, and easily available solvents could not only improve the efficiency of biosurfactant fermentation and but also decrease the recovery cost of biosurfactants.
机译：已经公知的是鼠李糖脂作为具有较高的生物降解性，低毒性潜力的天然生物表面活性剂和刺激性具有宽范围的在提高原油采收率，生物降解，生物修复和土壤洗涤潜在的应用。然而，大规模工业规模的生产鼠李糖脂的发泡在很大程度上抑制。在生物表面活性剂，快速搅拌和曝气大的发酵通常产生甚至在早期阶段并且这种泡沫产生泡沫的束是为随后的发酵一个严重的问题，由于污染，不稳定性，效率低。在本文中，铜绿假单胞菌ZJU被发酵用于通过使用废煎炸油作为唯一碳源产生鼠李糖脂。为了提高鼠李糖脂的生产效率，具有营养和微生物的恢复泡沫提取在一起的连续的集成系统引入发酵装置。在这种连续的集成系统，泡沫剂被允许通过外部环路流出生物反应器到收集容器用于通过与介质的组合物和微生物被泵送回发酵罐富含有机solvents.The水溶液提取鼠李糖脂。乙酸乙酯和乙醇以各种量和提取时间为例如使用被单独评估。据发现，通过乙醇泡沫提取大大增强鼠李糖脂的生产率至50g / L.Besides，当乙醇担任萃取剂鼠李糖脂下游处理被更容易地处理。总之，乙醇作为成本低，毒性更小，且容易获得的溶剂，不仅可以提高生物表面活性剂和发酵的效率，而且也降低生物表面活性剂的回收成本。

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