• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Academic Committee conference of Shanghai key lab of stomatology >ERBB2 gene amplification in oral squamous cell malignancies: a correlation with tumor progression and gene expression
【24h】

ERBB2 gene amplification in oral squamous cell malignancies: a correlation with tumor progression and gene expression

机译：口腔鳞状细胞恶性肿瘤中的ERBB2基因扩增：与肿瘤进展和基因表达的相关性

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objectives. Chromosomal instability is hallmark of carcinoma. Amplification of chromosome 17q11-q12 is present in some oral squamous cell cancer (OSCC) cases. In this study, we investigated the copy number variations of ERBB2 gene, which is located at this locus in collected OSCC samples and their correlation with tumor progression and gene expression. Study design. Quantitative real-time polymerase chain reaction was performed to detect the copy number of ERBB2 gene and the mRNA expression in 92 OSCC samples with matched adjacent normal tissues (ANTs). Proportional odds regression and 2-way repeated measurement analysis of variance were used to analyze the association between copy number variations and mRNA expression of the targeted gene. Results. Copy number gains of ERBB2 were detected in some of the OSCCs (19.6%, 18/92) and correlated with tumor stage (P < .001). Copy number gains of ERBB2 also showed a positive correlation with mRNA overexpression in OSCCs (P < .001). However, enhanced ERBB2 mRNA expression was also detected in a group of OSCC samples with unaltered copy number of ERBB2 gene (P < .05). Conclusions. Copy number increase of ERBB2 is observed in OSCCs and correlates with gene overexpression in these tumors. In addition, overexpression of ERBB2 is also observed in some OSCCs that lack copy number changes, indicating involvement of another mechanism.
机译：目标。染色体不稳定是癌的标志。染色体17Q11-Q12的扩增存在于一些口腔鳞状细胞癌（OSCC）病例中存在。在这项研究中，我们研究了ERBB2基因的拷贝数变异，其位于收集的OSCC样品中的该基因座及其与肿瘤进展和基因表达的相关性。学习规划。进行定量实时聚合酶链反应以检测ERBB2基因的拷贝数和92个OSCC样品中的mRNA表达，匹配相邻的正常组织（蚂蚁）。使用比例赔率回归和双向反复测量分析的方差分析综合数变异与靶基因的mRNA表达之间的关联。结果。在一些OSCC（19.6％，18/92）中检测到ERBB2的拷贝数量增益，并与肿瘤阶段相关（P <.001）。拷贝数量的ERBB2的收益还显示出与OSCC中的mRNA过表达的正相关（P <.001）。然而，在一组OSCC样品中也检测到增强的ERBB2 mRNA表达，其具有ERBB2基因的未嵌入拷贝数（P <.05）。结论。在OSCCS中观察到ERBB2的拷贝数增加，并与这些肿瘤中的基因过表达相关。此外，在一些缺乏拷贝数变化的OSCC中也观察到ERBB2的过表达，表明另一种机制的参与。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号