• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications >Magnetic field effects on spectrally-resolved lifetime of on-line oxygen monitoring using magneto-optic probes
【24h】

Magnetic field effects on spectrally-resolved lifetime of on-line oxygen monitoring using magneto-optic probes

机译：使用磁光探针对在线氧气监测的光谱分辨寿命的磁场效应

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Multimodal agents that serve as both probes for contrast and light-activated effectors of cellular processes in diseased tissue were developed. These agents were introduced into multicellular tumor spheroids (3D tissue models) and in the chorioallantoic membrane (CAM) of a chicken embryo. The luminescence decay was examined using a novel technique involving a spectrally-resolved fluorescence lifetime apparatus integrated with a weak electromagnet. A spectrallyresolved lifetime setup was used to identify magneto-optic species sensitive to magnetic field effects and distinguish from background emissions. We demonstrate that the applied magnetic fields can alter reaction rates and product distribution of some dyes detected by time- and spectrally-resolved luminescence changes. We will discuss the use of exogenous magneto-optical probes taken up in tumors to both induce phototoxicity, a process that is governed by complex and dynamically evolving mechanisms involving reactive oxygen species, and monitor treatment progress. The magnetic field enhancement, measured over a range of weak fields (0-300 mT) is correlated to oxygenation and may be used to monitor dynamic changes occurring due to oxygen consumption over the course of photodynamic therapy. Such online measurements provide the possibility to derive real-time information about response to treatment via monitoring magnetic field enhancement/suppression of the time-resolved, spectrally-resolved luminescence of the probe at the site of the treatment directly. Magnetic perturbation of lifetime can serve as a status reporter, providing optical feedback of oxygen-mediated treatments in situ and allowing for real-time adjustment of a phototherapy treatment plan.
机译：发育了一种作为患病组织中细胞过程对比和光活性效应探针的多峰剂。将这些试剂引入多细胞肿瘤球状体（3D组织模型）和鸡胚的胆管囊膜（凸轮）中。使用涉及与弱电磁铁集成的光谱分辨的荧光寿命装置进行了一种新技术来检查发光衰减。光谱稳定的寿命设置用于识别对磁场效应敏感的磁光物种，并区分背景排放。我们证明所应用的磁场可以改变通过时间和光谱分辨发光的一些染料的反应速率和产物分布。我们将讨论在肿瘤中造成的外源磁光探针对诱导的光毒性，一种由涉及反应性氧物种的复杂和动态演化机制来治理的过程，以及监测治疗进展。在一系列弱场（0-300mT）上测量的磁场增强与氧合有关，可用于监测由于光动力疗法过程中由于氧气消耗而发生的动态变化。这种在线测量提供了通过监测磁场增强/抑制在治疗部位的时间分辨的，可分辨的探针的时间分辨的探针的探测器的磁场增强/抑制来获得关于治疗的响应的实时信息的可能性。寿命的磁性扰动可以用作状态报告器，提供原位的氧介导治疗的光反馈并允许实时调节光疗处理计划。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号