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Synthesis of Zinc Oxide Nanoparticles and Their Antibacterial Activity

机译:氧化锌纳米粒子的合成及其抗菌活性

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Zinc oxide nanoparticles (ZnO NPs) have received significant interest in bioengineering. Herein, ZnO NPs synthesis was performed via sol-gel method using precursors including zinc acetate salt and sodium hydroxide, followed by calcination of precipitated part at 180°C. The synthesized ZnO NPs properties in terms of phase composition, size and shape were then characterized by X-ray diffraction (XRD), ultraviolet–visible spectroscopy (UV-vis) and transmission electron microscopy (TEM). To test antibacterial ability of the synthesized ZnO NPs, glass pieces were covered with suspension containing different concentration of ZnO NPs synthesized, afterwards put into Escherichia coli (E.coli) culture after 24 hours of incubation at 37°C. The synthesized ZnO NPs characterized by TEM were mainly rod shape with particles size ranging 30-100 nm; moreover, UV-vis spectrum of the ZnO NPs suspension presented an absorbance peak at 371nm. Furthermore, antibacterial activity of them assessed throughout visualization of E. coli live was found to be influenced on dosed used.
机译:氧化锌纳米颗粒(ZnO NPS)对生物工程产生了显着的兴趣。在此,通过使用包括乙酸盐和氢氧化钠的前体的溶胶 - 凝胶法进行ZnO NPS合成,然后在180℃下煅烧沉淀部分。在相组成,尺寸和形状方面的合成氧化锌纳米粒子的属性然后通过X射线衍射(XRD),紫外可见分光法(UV-VIS)和表征透射电子显微镜(TEM)。为了测试合成的ZnO NPS的抗菌能力,用含有不同浓度的ZnO NPS合成的悬浮液覆盖玻璃碎片,然后在37℃温育24小时后进入大肠杆菌(例如COLI)培养物。由TEM表征的合成ZnO NP主要是杆状,粒子尺寸范围为30-100nm;此外,ZnO NPS悬浮液的UV-Vis光谱呈现在371nm的吸光度峰。此外,发现在整个E.Coli Live的可视化中评估它们的抗菌活性受到使用的剂量的影响。

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