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Research of Separation of Amino Acids on Micro-Free Flow Electrophoresis Chip with Voltage Applied in Two-Dimension

机译:二维电压微自由流电泳芯片上氨基酸分离的研究

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摘要

The Free Flow Electrophoresis micro Chip with voltage applied in two-dimension and coupled with hydraulic control unit was designed in this paper, which can achieve fluid transport and sample separation in the electrophoresis process. The Alpha gel scanning imaging system was used as the detector, which could observe experiment phenomenon, record and analyse experiment results. Under the optimized conditions of the 10mmol borate buffer contain 3 %( v/v) glycerol, at pH 10 and the ratio of field strength in two-dimension at 1:5, the mixed amino acids including phenylalanine (L-Phe) and lysine (L-Lys) were successfully separated in the μ-FFE analytical system. The experiment had shown that the correlation coefficient of the L-Phe and L-Lys was up to 0.993 and 0.992, respectively. The results illustrated that u-FFE have showed great potential perspective in the separation of mix Amino Acids, especially in the quantitative test of the trace Amino Acids in clinic samples.
机译:本文设计了二维自由电压电泳并结合液压控制单元的电泳微芯片,可以实现电泳过程中的流体传输和样品分离。以Alpha凝胶扫描成像系统为检测器,可以观察实验现象,记录并分析实验结果。在10mmol硼酸盐缓冲液的优化条件下,在pH 10时含有3%(v / v)的甘油,二维场强比为1:5,混合氨基酸包括苯丙氨酸(L-Phe)和赖氨酸(L-Lys)在μ-FFE分析系统中成功分离。实验表明,L-Phe和L-Lys的相关系数分别高达0.993和0.992。结果表明,u-FFE在混合氨基酸的分离中,特别是在临床样品中痕量氨基酸的定量测试中,具有广阔的前景。

著录项

  • 来源
  • 会议地点 Xian(CN);Xian(CN)
  • 作者单位

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University,International RD Center of Micro-Nano Systems and New Materials Technology, Chongqing University;

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University,International RD Center of Micro-Nano Systems and New Materials Technology, Chongqing University,Micro system Research Center, Chongqing University, Chongqing 400030, China;

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University,International RD Center of Micro-Nano Systems and New Materials Technology, Chongqing University;

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University,International RD Center of Micro-Nano Systems and New Materials Technology, Chongqing University;

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University;

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University,International RD Center of Micro-Nano Systems and New Materials Technology, Chongqing University;

    Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 光学仪器;微电子学、集成电路(IC);
  • 关键词

    two-dimensional electrophoresis microchip; FFE; hydraulic control; the separation of amino acids;

    机译:二维电泳微芯片FFE;液压控制;氨基酸分离;

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