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首页> 外文会议>Microfluidics, BioMEMS, and Medical Microsystems IV >Fully integrated multiplexed lab-on-a-card assay for enteric pathogens
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Fully integrated multiplexed lab-on-a-card assay for enteric pathogens

机译：完全整合的多重卡式实验室化验法，用于肠道病原体

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摘要

Under this NIH-funded project, we are developing a lab-on-a-card platform to identify enteric bacterial pathogens in patients presenting with acute diarrhea, with special reference to infections that might be encountered in developing countries. Component functions that are integrated on this platform include on-chip immunocapture of live or whole pathogens, multiplexed nucleic acid amplification and on-chip detection, sample processing to support direct use of clinical specimens, and dry reagent storage and handling. All microfluidic functions are contained on the lab card. This new diagnostic test will be able to rapidly identify and differentiate Shigella dysenteriae serotype 1, Shigella toxin-producing Escherichia coli, E. coli 0157, Campylobacter jejuni, and Salmonella and Shigella species. This presentation will report on progress to date on sample and bacteria processing methodologies, identification and validation of capture antibodies and strategy for organism immunocapture, identification and validation of specific polymerase chain reaction (PCR) primer sequences for over 200 clinical isolates of enteric pathogens, and implementation of on-chip nucleic acid extraction for a subset of those pathogens.
机译：在这个由美国国立卫生研究院资助的项目下，我们正在开发一个卡片实验室平台，以识别急性腹泻患者的肠道细菌病原体，并特别提及发展中国家可能遇到的感染。该平台上集成的组件功能包括活体或完整病原体的片上免疫捕获，多重核酸扩增和片上检测，支持直接使用临床标本的样品处理以及干试剂的存储和处理。所有微流体功能均包含在实验卡中。这项新的诊断测试将能够快速鉴定和区分痢疾志贺氏菌血清型1，产志贺氏菌毒素的大肠杆菌，大肠杆菌0157，空肠弯曲菌，沙门氏菌和志贺氏菌。本演讲将报告样品和细菌加工方法的最新进展，捕获抗体的鉴定和验证以及生物体免疫捕获的策略，200多种肠道病原体临床分离株的特异性聚合酶链反应（PCR）引物序列的鉴定和验证，以及对这些病原体的子集进行芯片上核酸提取。

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