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首页> 外文会议>Microfluidics, BioMEMS, and Medical Microsystems V; Proceedings of SPIE-The International Society for Optical Engineering; vol.6465 >An Integrated Microsystem for Multiplex Processing of Encoded Silicon Microbeads
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An Integrated Microsystem for Multiplex Processing of Encoded Silicon Microbeads

机译：用于编码硅微珠的多重处理的集成微系统

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摘要

A novel integrated microsystem for multiplex processing of encoded microbeads on a single microchip is presented. Conventional bio-analysis of proteins and DNA requires a combination of different techniques including: accurate delivery of reagents, mixing, then reaction at controlled temperature to yield a detectable product. Standard laboratory bio-assays require intervention at several stages to manipulate samples. Furthermore, ultra sensitive quantification with a colorimetric or fluorescent label is required to obtain the necessary results. This process is time consuming and labour intensive. However the new multiplex microsystem reported here enhances logical bio-assay development due to the integration of a compact optical detection system with customized analysis software in an enclosed microfluidic environment. The bioassay was realised by careful integration of a Peltier cell and associated control electronics which enabled specific identification of a hybridized DNA sequence from a 4 × 4 cDNA library at a fixed temperature of 42℃. The multiple fluorescence measurements of complementary hybridised DNA at the surface of encoded microbeads was confirmed. Thus, the complete integration of the different bio-assay components on a single multiplex assay platform provides distinct advantages of reduced sample volume, rapid analysis and low cost.
机译：提出了一种新颖的集成微系统，用于在单个微芯片上对编码微珠进行多重处理。蛋白质和DNA的常规生物分析需要多种不同技术的结合，包括：准确地输送试剂，混合，然后在受控温度下反应以产生可检测的产物。标准实验室生物测定需要在多个阶段进行干预才能处理样品。此外，需要使用比色或荧光标记物进行超灵敏定量以获得必要的结果。此过程既费时又费力。但是，由于在封闭的微流体环境中集成了紧凑的光学检测系统和定制的分析软件，因此本文报道的新的多重微系统增强了逻辑生物测定的发展。该生物测定是通过仔细整合Peltier细胞和相关的控制电子器件而实现的，该电子器件可在42℃的固定温度下从4×4 cDNA文库中特异性鉴定杂交的DNA序列。互补的杂交DNA在编码的微珠表面的多次荧光测量得到证实。因此，将不同生物测定成分完全整合在单个多重测定平台上可提供减少样品量，快速分析和低成本的独特优势。

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