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Fourier transform infrared microspectroscopy shows significant differences between spectra of undifferentiated and polynucleated FLG 29.1 dried cells

机译:傅立叶变换红外光谱显示未分化和多核FLG 29.1干细胞的光谱之间存在显着差异

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Abstract: In a recent study made on cultures of human leukaemic cells (FLG 29.1 cell line) we were able to detect, by IR microspectroscopy, some significant IR spectroscopic variations following differentiation of cells towards osteoclastic-like behavior. The present study was undertaken on the same cell line in order to monitor biochemical structure variations following fusion induced by polyetilenglycole (PEG), using FTIR microspectroscopy. The finger-print region of all the spectra was retained and normalized according to a new regression procedure. Eleven bands were selected and total band power and mean power per unit frequency were compared with the corresponding reference session bands by a Dunnett's T test. Significant differences were found in both the tested variables only between treated and untreated cells, in 6 bands. !12
机译:摘要:在一项关于人类白血病细胞(FLG 29.1细胞系)培养物的最新研究中,我们能够通过红外显微术检测到一些细胞分化为破骨细胞样行为后的一些明显的红外光谱变化。本研究是在同一细胞系上进行的,目的是使用FTIR显微技术监测聚乙二醇(PEG)诱导融合后的生化结构变化。保留所有光谱的指纹区域,并根据新的回归程序进行归一化。选择了11个频段,并通过Dunnett T检验将总频段功率和每单位频率的平均功率与相应的参考会话频段进行了比较。仅在经处理的和未经处理的细胞之间,在6个条带中,在两个测试变量中都发现了显着差异。 !12

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