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首页> 外文会议>Multiphoton microscopy in the biomedical sciences IX >In-vivo and in-vitro investigations of retinal fluorophores in age-related macular degeneration by fluorescence lifetime imaging
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In-vivo and in-vitro investigations of retinal fluorophores in age-related macular degeneration by fluorescence lifetime imaging

机译：通过荧光寿命成像对年龄相关性黄斑变性中视网膜荧光团的体内和体外研究

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摘要

Ocular fundus autofluorescence imaging has been introduced into clinical diagnostics recently for the observation of the age pigment lipofuscin, a precursor of age - related macular degeneration (AMD). However, a deeper understanding of the generation of single compounds contributing to the lipofuscin as well as of the role of other fluorophores such as FAD, glycated proteins, and collagen needs their discrimination by fluorescence lifetime imaging (FLIM).rnFLIM at the ocular fundus is performed using a scanning laser ophthalmoscope equipped with a picosecond laser source (448nm or 468nm respectively, 100ps, 80 MHz repetition rate) and dual wavelength (490-560nm and 560-7600nm) time-correlated single photon counting. A three - exponential fit of the fluorescence decay revealed associations of decay times to anatomical structures. Disease - related features are identified from alterations in decay times and-amplitudes.rnThe in-vivo investigations in patients were paralleled by experiments in an organ culture of the porcine ocular fundus Photo - oxidative stress was induced by exposure to blue light (467nm, 0.41 mW/mm∧2). Subsequent analysis (fluorescence microscopy, HPLC, LC-MS) indicated the accumulation of the pyridinium bis-retinoid A2E and its; oxidation products as well as oxidized phospholipids. These compounds contribute to the tissue auto - fluorescence and may play a key role in the pathogenesis of AMD. Thus, FLIM observation at the ocular fundus in vivo enhances our knowledge on the etiology of AMD and may become a diagnostic tool.
机译：最近，眼底自体荧光成像已被引入临床诊断中，用于观察年龄相关性黄斑变性（AMD）的前体年龄色素脂褐素。但是，对于形成脂褐素的单个化合物以及其他荧光团（如FAD，糖基化蛋白和胶原蛋白）的作用的更深入了解，需要通过荧光寿命成像（FLIM）加以区分。使用装有皮秒激光源（分别为448nm或468nm，100ps，80 MHz重复频率）和双波长（490-560nm和560-7600nm）时间相关的单光子计数的扫描激光检眼镜进行。荧光衰减的三指数拟合显示了衰减时间与解剖结构的关联。通过衰减时间和振幅的变化来确定与疾病相关的特征。rn-通过在猪眼底器官培养物中进行的实验与患者体内研究相平行。通过暴露于蓝光（467nm，0.41）诱发光氧化应激mW /mm∧2）。随后的分析（荧光显微镜，HPLC，LC-MS）表明吡啶鎓双类视黄醇A2E及其积累。氧化产物以及氧化的磷脂。这些化合物有助于组织自发荧光，并可能在AMD的发病机理中起关键作用。因此，在体内眼底进行FLIM观察增强了我们对AMD病因的认识，并可能成为诊断工具。

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  • 会议地点 San Jose CA(US)
  • 作者

    M. Hammer; S. Quick; M. Klemm; S. Schenke; N. Mata; A. Eitner; D. Schweitzer;

  • 作者单位

    Univ. of Jena, Department of Ophthalmology, Bachstr. 18, 07740 Jena, Germany;

    Univ. of Jena, Department of Ophthalmology, Bachstr. 18, 07740 Jena, Germany;

    Technical Univ. Ilmenau, Computer Science and Automation Department, Institute of Biomedical Engineering and Informatics, POB 100565, 98684 Ilmenau, Germany;

    Univ. of Jena, Department of Ophthalmology, Bachstr. 18, 07740 Jena, Germany;

    SIRiON Therapeutics Inc. 11408 Sorrento Valley Rd, San Diego, CA 92121. U.S.A.;

    Univ. of Jena, Institute of Anatomy II, Teichgraben 7, 07740 Jena, Germany;

    Univ. of Jena, Department of Ophthalmology, Bachstr. 18, 07740 Jena, Germany;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    age-related macular degeneration; native fluorescence; FLIM; organ culture;

    机译：年龄相关性黄斑变性;天然荧光FLIM;器官文化;

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