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首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VII; Progress in Biomedical Optics and Imaging; vol.8 no.19; Proceedings of SPIE-The International Society for Optical Engineering; vol.6442 >Coherent anti-Stokes Raman scattering microscope with a high signal-to-noise ratio, high stability, and high-speed imaging for live cell observation
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Coherent anti-Stokes Raman scattering microscope with a high signal-to-noise ratio, high stability, and high-speed imaging for live cell observation

机译：相干抗斯托克斯拉曼散射显微镜，具有高信噪比，高稳定性和用于活细胞观察的高速成像

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Coherent anti-Stokes Raman scattering (CARS) microscopy, which can produce images of specific molecules without staining, has attracted the attention of researchers, as it matches the need for molecular imaging and pathway analysis of live cells. In particular, there have been an increasing number of CARS experimental results regarding lipids in live cells, which cannot be fluorescently tagged while keeping the cells alive. One of the important applications of lipid research is for the metabolic syndrome. Since the metabolic syndrome is said to be related to the lipids in lipocytes, blood, arterial vessels, and so on, the CARS technique is expected to find application in this field. However, CARS microscopy requires a pair of picosecond laser pulses, which overlap both temporally and spatially. This makes the optical adjustments of a CARS microscope challenging. The authors developed a CARS unit that includes optics for easy and stable adjustment of the overlap of these laser pulses. Adding the CARS unit to a laser scanning microscope provides CARS images of a high signal-to-noise ratio, with an acquisition rate as high as 2 microseconds per pixel. Thus, images of fast-moving lipid droplets in Hela cells were obtained.
机译：相干抗斯托克斯拉曼散射（CARS）显微镜可以产生特定分子的图像而无需染色，因此吸引了研究人员的注意力，因为它符合对活细胞进行分子成像和途径分析的需要。特别是，关于活细胞中脂质的CARS实验结果越来越多，这些脂质在保持细胞存活的同时不能被荧光标记。脂质研究的重要应用之一是代谢综合征。由于据称代谢综合征与脂肪细胞，血液，动脉血管等中的脂质有关，因此CARS技术有望在该领域中得到应用。但是，CARS显微镜需要一对皮秒激光脉冲，它们在时间和空间上都重叠。这使CARS显微镜的光学调节变得困难。作者开发了一种CARS单元，其中包括用于轻松稳定地调节这些激光脉冲重叠的光学元件。将CARS单元添加到激光扫描显微镜后，可提供高信噪比的CARS图像，每像素的采集速率高达2微秒。因此，获得了Hela细胞中快速移动的脂质滴的图像。

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