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Fluorescence lifetime imaging microscopy using a streak camera

机译:使用条纹相机的荧光寿命成像显微镜

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摘要

We present the development of a fluorescence lifetime imaging microscopy system using a streak camera (SC-FLIM), which uses ultrafast infrared laser for multiphoton excitation and a streak camera for lifetime measurement. A pair of galvo mirrors are employed to accomplish quick time-resolved scanning on a line and 2D fluorescence lifetime imaging. The SC-FLIM system was calibrated using an F-P etalon and several standard fluorescent dyes, and was also used to perform fluorescence lifetime imaging of fluorescent microspheres and a prepared plant stem slide.
机译:我们介绍了使用条纹相机(SC-FLIM)的荧光寿命成像显微系统的开发,该系统使用超快红外激光进行多光子激发,并使用条纹相机进行寿命测量。一对检流镜可用于完成在线上快速的时间分辨扫描和2D荧光寿命成像。使用F-P标准具和几种标准荧光染料对SC-FLIM系统进行校准,并且还用于对荧光微球和制备的植物茎片进行荧光寿命成像。

著录项

  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者单位

    School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, China;

    School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, China;

    School of Physics and Optoelectronic Engineering, Xidian University, Xi'an 710071, China;

    Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China;

    Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China;

    Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fluorescence lifetime imaging microscopy (FLIM); streak camera; two-photon excitation fluorescence;

    机译:荧光寿命成像显微镜(FLIM);条纹相机双光子激发荧光;

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