• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Multiphoton microscopy in the biomedical sciences XVII >Selection of proper objective lens for the higher-order multiphoton microscopy at the 1700-nm window
【24h】

Selection of proper objective lens for the higher-order multiphoton microscopy at the 1700-nm window

机译：在1700 nm窗口为高阶多光子显微镜选择合适的物镜

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 1700-nm window has emerged as a promising excitation window for multiphoton microscopy (MPM). On one hand,the combined low tissue absorption and scattering make this window well suited for deep-tissue MPM; on the other hand, the long excitation wavelength makes higher-order MPM in biological tissues feasible, e.g., recently 4-photon fluorescence MPM in mouse brain has been demonstrated. Objective lens is a key optical component in the entire MPM setup. Multiphoton signal levels are largely dependent on the transmittance of objective lens. Here we demonstrate experimental results of transmittance measurement of two water immersion objective lenses commonly used for MPM at the 1700-nm window, covering both the excitation and the signal window. Our target application is MPM of even higher order excited at this window, i.e., 4th harmonic generation (FHG) imaging and 5-photon fluorescence generation. Our results show that, although the customized objective lens offers higher transmittance at the excitation window, it suffers from dramatically degraded transmittance at the signal window, compared with the non-customized objective lens. These results will offer guidelines for selection of proper objective lens for higher-order MPM at the 1700-nm window.
机译：1700 nm的窗口已经成为多光子显微镜（MPM）的有希望的激发窗口。一方面，较低的组织吸收和散射相结合，使该窗口非常适合于深部组织的MPM。另一方面，长激发波长使生物组织中更高阶的MPM成为可能，例如，最近已证明小鼠脑中的4光子荧光MPM。物镜是整个MPM设置中的关键光学组件。多光子信号电平在很大程度上取决于物镜的透射率。在这里，我们演示了两个通常用于MPM的水浸式物镜在1700 nm窗口下的透射率测量的实验结果，该窗口同时覆盖了激发和信号窗口。我们的目标应用是在此窗口激发更高阶的MPM，即4次谐波生成（FHG）成像和5光子荧光生成。我们的结果表明，尽管定制的物镜在激发窗口处提供更高的透射率，但与非定制的物镜相比，它在信号窗口处的透射率急剧下降。这些结果将为在1700 nm窗口中选择合适的物镜用于高阶MPM提供指导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号