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Comparison of the Microbial Community Composition at Yucca Mountain andLaboratory Test Nuclear Repository Environments

机译:丝兰山和实验室测试核储存库环境中微生物群落组成的比较

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The microbiological community structure within a proposed nuclear waste repository at Yucca Mountain(YM), NV was determined. Microbial growth from collected rock was detected using simulated groundwater as a growth medium, with or without amendment of a carbon source. Grown isolates wereidentified by 16S ribosomal DNA (rDNA) sequence analysis. A more complete compositional analysis ofthe microbial community located at the proposed nuclear waste repository site was performed usingenvironmental DNA isolation and subsequent identification of amplified 16S rDNA genes. Concurrently,a series of corrosion testing tanks that simulate the evolution of anticipated environmental conditionswithin the proposed repository have been subjected to the same type of analyses.
机译:确定了内华达州尤卡山(YMca)拟建核废料库中的微生物群落结构。使用模拟地下水作为生长介质,无论是否添加碳源,都可以检测收集到的岩石中的微生物。通过16S核糖体DNA(rDNA)序列分析鉴定了生长的分离株。使用环境DNA分离和随后的扩增16S rDNA基因鉴定,对位于拟议的核废料处置场所的微生物群落进行了更完整的组成分析。同时,在拟议的储存库中模拟预期环境条件演变的一系列腐蚀试验箱已经进行了相同类型的分析。

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