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SHARP GOLD BASED HYBRID NANOPROBES FOR CELL IMAGING THROUGH DARK-FIELD MICROSCOPY

机译:夏普金基混合纳米探针,用于通过暗场显微镜对细胞成像

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摘要

Numerous works are based on the design, the elaboration and the study of the optical properties of gold nanoprobes for potential applications in biotechnologies (bioimaging, biosensing). Among all the possible shapes, it appears that sharp gold nanostructures exhibit interesting features due to the strong filed generated at their tips ends, making them very sensitive to the surrounding medium. Here we describe a complete study of PEGylated gold nanoparticles : nanostars and bipyramids as potential agents for bioimaging. The nanoprobes are first prepared in high yield before functionalization with a biocompatible polymer. Then, the PEGylated gold nanoparticles are incubated with melanoma B16-F10 cells and observed using Dark-field microscopy. Results show that the biocompatible gold nanoparticles are easily internalized and most of them localized within the cells.
机译:基于金纳米探针在生物技术(生物成像,生物传感)中的潜在应用的设计,阐述和光学特性的研究,许多工作都是基于此。在所有可能的形状中,尖锐的金纳米结构似乎表现出有趣的特征,这归因于其尖端产生的强电场,使其对周围的介质非常敏感。在这里,我们描述了对聚乙二醇化金纳米颗粒的完整研究:纳米星和双锥体作为生物成像的潜在媒介。在用生物相容性聚合物官能化之前,首先以高产率制备纳米探针。然后,将聚乙二醇化的金纳米颗粒与黑色素瘤B16-F10细胞孵育,并使用暗视野显微镜观察。结果表明,生物相容性金纳米颗粒易于内化,并且大多数位于细胞内。

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  • 来源
  • 会议地点 San Diego CA(US)
  • 作者单位

    Universite de Lyon, ENS Lyon, CNRS, universite Lyon 1, Laboratoire de Chimie UMR 5182, 46, allee d'Italie, F-69364, Lyon cedex 07, France;

    Universite de Lyon, ENS Lyon, CNRS, universite Lyon 1, Laboratoire de Chimie UMR 5182, 46, allee d'Italie, F-69364, Lyon cedex 07, France;

    Universite de Lyon, ENS Lyon, CNRS, universite Lyon 1, Laboratoire de Chimie UMR 5182, 46, allee d'Italie, F-69364, Lyon cedex 07, France;

    Universite de Lyon, ENS Lyon, CNRS, universite Lyon 1, Laboratoire de Chimie UMR 5182, 46, allee d'Italie, F-69364, Lyon cedex 07, France;

    Universite de Lyon, ENS Lyon, CNRS, universite Lyon 1, Laboratoire de Chimie UMR 5182, 46, allee d'Italie, F-69364, Lyon cedex 07, France;

    Universite de Lyon, ENS Lyon, CNRS, universite Lyon 1, Laboratoire de Chimie UMR 5182, 46, allee d'Italie, F-69364, Lyon cedex 07, France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Gold; bipyramids; stars; plasmon; single particle spectroscopy; cells imaging;

    机译:金;双锥星星;等离子体激元单粒子光谱;细胞成像;

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