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首页> 外文会议>Nanomaterial Synthesis and Integration for Sensors, Electronics, Photonics, and Electro-Optics; Proceedings of SPIE-The International Society for Optical Engineering; vol.6370 >Ordered DNA Arrays Prepared Via Soft Lithography
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Ordered DNA Arrays Prepared Via Soft Lithography

机译：通过软光刻制备的有序DNA阵列

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This paper reports progress in an approach to create a general purpose platform to be used in the reproducible assembly of molecular electronic devices. We describe a method in which DNA molecules were immobilized on patterned neutravidin surfaces. First, a silicon wafer was functionalized with (3-aminopropyl)triethoxysilane (APTES) to produce an amine-terminated surface. The primary amine group was then reacted with the heterobifunctional linker molecule succinimidyl-6-(biotinamido)hexanoate which placed an active biotin group at the surface interface. These biotinylated surfaces were then patterned with the tetrameric protein neutravidin using microcontact printing (μCP) with relief features in polydimethylsiloxane (PDMS) stamps. The neutravidin proteins adsorb onto the surface and bind nearly irreversibly to one or two biotin groups leaving at least two biotin binding sites on each protein available for conjugation. Following neutravidin stamping, 1 μm long DNA molecules functionalized on one end with biotin were attached to the patterned areas. Water contact angle (WCA) measurements were used to characterize wettability changes of the silicon surfaces for amine and biotin functionalization. Atomic force microscopy (AFM) was used to image the patterns of immobilized neutravidin and DNA.
机译：本文报告了一种创建通用平台的方法的进展，该平台可用于可重复组装的分子电子设备。我们描述了一种方法，其中DNA分子固定在图案化的中性亲和素表面上。首先，将硅晶片用（3-氨基丙基）三乙氧基硅烷（APTES）进行功能化，以生成胺端基表面。然后使伯胺基与异双功能接头分子琥珀酰亚胺-6-（生物素亚氨基）己酸酯反应，将活性生物素基团置于表面界面。然后使用具有聚二甲基硅氧烷（PDMS）压印凸版特征的微接触印刷（μCP），用四聚体蛋白中性亲和素对这些生物素化的表面进行构图。中性亲和素蛋白吸附到表面上并几乎不可逆地结合到一个或两个生物素基团上，在每个蛋白质上至少有两个生物素结合位点可用于结合。在中性亲和素冲压后，将一端用生物素功能化的1μm长DNA分子附着到图案区域。水接触角（WCA）测量用于表征胺和生物素功能化的硅表面的润湿性变化。原子力显微镜（AFM）用于对固定的中性亲和素和DNA的图像进行成像。

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