Patterning of fibronectin using laser writer for force measurement in cells

机译：使用激光写入器对纤连蛋白进行构图以测量细胞中的力

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Mechanical forces and living cells are closely related, in particular in connections, named Focal Adhesions (FAs),rnbetween cells and extracellular matrix. FAs are mechanosensors and regulate physiological processes.rnThe aim of this contribution is to explore the possibility of performing micrometric and submicrometric protein patternrnin order to study FAs on different materials. Typical substrates for microelectromechanical systems (MEMS) are testedrnand the results are reported.rnA laser is used to produce a pattern of extracellular matrix proteins, like fibronectin. A new use of a Raman microprobernis described. Cells arrange in a regular shape, following the geometry of the pattern. Protein spots last for more than 24rnhours.rnIt is very important to have a complete control on FAs and the technique proposed is a suitable solution.

机译：机械力与活细胞密切相关，特别是在细胞与细胞外基质之间的连接上，即所谓的粘着力（FAS）。 FAs是机械传感器，可调节生理过程。此贡献的目的是探索执行微米和亚微米级蛋白质模式的可能性，以便研究不同材料上的FAs。测试了微机电系统（MEMS）的典型基板，并报告了结果。使用激光产生细胞外基质蛋白（如纤连蛋白）的图案。描述了拉曼微探针的新用途。单元按照图案的几何形状以规则的形状排列。蛋白质斑点持续超过24rnhours。rn对FA进行完全控制非常重要，建议的技术是合适的解决方案。

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《Nanoscale imaging, sensing, and actuation for biomedical applications VI》|2009年|71880P.1-71880P.8|共8页
会议地点 San Jose CA(US)
作者
B. Codan; T. Gaiotto; R. Di Niro; R. Marzari; V. Sergo;
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作者单位

Department of Materials and Natural Resources, University of Trieste, Trieste, Italy;

Biology Dept., University of Trieste, Trieste, Italy;

Institute of Immunology, Rikshospitalet University of Oslo, Norway;

Department of Materials and Natural Resources, University of Trieste, Trieste, Italy;

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原文格式 PDF
正文语种 eng
中图分类
关键词
focal adhesions; proteins patterning; laser writer; raman lithography;

机译：粘连蛋白质模式；激光刻录机拉曼光刻;

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专利

1. Quantitative evaluation of cell attachment to glass, polystyrene, and fibronectin- or collagen-coated polystyrene by measurement of cell adhesive shear force and cell detachment energy. [J] . Yamamoto A, Mishima S, Maruyama N, Journal of biomedical materials research. Part B, Applied biomaterials. . 2000,第2期

机译：通过测量细胞粘附剪切力和细胞分离能，定量评估细胞与玻璃，聚苯乙烯和纤连蛋白或胶原蛋白包被的聚苯乙烯的附着力。
2. SHORT PAPER: Atomic force microscopy measurements of mechanical properties of single cells patterned by microcontact printing [J] . Ryosuke Takahashi, Satoshi Ichikawa, Agus Subagyo, Advanced Robotics: The International Journal of the Robotics Society of Japan . 2014,第7a8期

机译：简短论文：原子力显微镜测量通过微接触印刷形成图案的单细胞的机械性能
3. Fibronectin fibril pattern displays the force balance of cell-matrix adhesion [J] . Pompe T, Keller K, Mitdank C, European Biophysics Journal . 2005,第8期

机译：纤连蛋白的原纤维形态显示细胞-基质粘附的力平衡
4. Patterning of fibronectin using laser writerfor force measurement in cells [C] . B. Codan, T. Gaiotto, R. Di Niro, Conference on nanoscale imaging, sensing, and actuation for biomedical applications . 2009

机译：使用激光作理迫使细胞测量纤连蛋白的图案化
5. An atomic force microscopy study of polycaprolactone biomaterial surface characterization and adhesion measurements between fibronectin and human alpha(5)beta(1) integrins [D] . Markowitz, Sharon 2008

机译：聚己内酯生物材料表面表征和纤连蛋白与人alpha（5）beta（1）整合素之间的粘附力测量的原子力显微镜研究
6. Intracellular forces during guided cell growth on micropatterns using FRET measurement [O] . Kevin Suffoletto, Nannan Ye, Fanjie Meng, -1

机译：使用FRET测量在微模式下引导细胞生长期间的细胞内力
7. Laser safety evaluation and output measurements for the VITAL -2 Variable Intensity Tactical Aiming Light (laser) used with the Proforce M-4 system in force-on-force exercises. [O] . Augustoni, Arnold L. 2004

机译：Vower -2可变强度战术瞄准灯（激光）的激光安全评估和输出测量与proforce m-4系统一起使用，用于力量练习。

Patterning of fibronectin using laser writer for force measurement in cells

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