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首页> 外文会议>Nanoscale imaging, sensing, and actuation for biomedical applications XI >Role of nonspecific binding: a comparison among flow through and flow over assays in nanoporous material
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Role of nonspecific binding: a comparison among flow through and flow over assays in nanoporous material

机译：非特异性结合的作用：纳米多孔材料中流通和流通分析之间的比较

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In this article we describe the fabrication of free standing n-type porous silicon microcavity (MC) and their properties as liquid sensors. We have optimized the etching recipe to keep both large pore size and high quality factor (Q-factor). Thus the fabricated porous layers have pore size in the range of 40 to 110 nm and are thus compatible with mass transport across the porous layer. We found that MC with a Q-factor of 60 can measure down to 1.1~*10~(-5) refractive index variations. Furthermore we analyze the role of non specific binding by comparing flow through versus flow over geometries. We compare these two approaches using different techniques and we show that flow over assay systematically overestimates the sensitivity of the device because of an inefficient rinse of the sample. Our work clearly indicates a limit in the reliability of measurements performed in flow over geometry unless specific controls are taken into account.
机译：在本文中，我们描述了独立式n型多孔硅微腔（MC）的制造及其作为液体传感器的特性。我们优化了蚀刻配方，以保持较大的孔径和高质量因子（Q因子）。因此，所制造的多孔层具有在40至110nm范围内的孔径，并因此与穿过多孔层的质量传递相容。我们发现Q值为60的MC可以测量低至1.1〜* 10〜（-5）的折射率变化。此外，我们通过比较流过的几何形状与流过的几何形状来分析非特异性结合的作用。我们使用不同的技术对这两种方法进行了比较，结果表明，由于样品冲洗效率低，流过检测系统地高估了设备的灵敏度。我们的工作清楚地表明，除非考虑特定的控制措施，否则在几何形状上进行的测量的可靠性受到限制。

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