• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Nanoscale imaging, sensing, and actuation for biomedical applications XI >Imaging Mesenchymal Stem Cells Containing Single Wall Nanotube Nanoprobes In a 3D Scaffold Using Photo-Thermal Optical Coherence Tomography
【24h】

Imaging Mesenchymal Stem Cells Containing Single Wall Nanotube Nanoprobes In a 3D Scaffold Using Photo-Thermal Optical Coherence Tomography

机译：使用光热光学相干断层扫描成像技术在3D支架中包含单壁纳米管纳米探针的间充质干细胞

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Despite the fact, that a range of clinically viable imaging modalities, such as magnetic resonance imaging (MRI), computed tomography (CT), photo emission tomography (PET), ultrasound and bioluminescence imaging are being optimised to track cells in vivo, many of these techniques are subject to limitations such as the levels of contrast agent required, toxic effects of radiotracers, photo attenuation of tissue and backscatter. With the advent of nanotechnology, nanoprobes are leading the charge to overcome these limitations. In particular, single wall nanotubes (SWNT) have been shown to be taken up by cells and as such are effective nanoprobes for cell imaging. Consequently, the main aim of this research is to employ mesenchymal stem cells (MSC) containing SWNT nanoprobes to image cell distribution in a 3D scaffold for cartilage repair. To this end, MSC were cultured in the presence of 32μg/ml SWNT in cell culture medium (αMEM, 10% FBS, 1% penicillin/streptomycin) for 24 hours. Upon confirmation of cell viability, the MSC containing SWNT were encapsulated in hyaluronic acid gels and loaded on polylactic acid polycaprolactone scaffolds. After 28 days in complete chondrogenic medium, with medium changes every 2 days, chondrogenesis was confirmed by the presence of glycosaminoglycan. Moreover, using photothermal optical coherence tomography (PT-OCT), the cells were seen to be distributed through the scaffold with high resolution. In summary, these data reveal that MSC containing SWNT nanoprobes in combination with PT-OCT offer an exciting opportunity for stem cell tracking in vitro for assessing seeding scaffolds and in vivo for determining biodistribution.
机译：尽管事实如此，但仍在优化一系列临床可行的成像方式，例如磁共振成像（MRI），计算机断层扫描（CT），光发射断层扫描（PET），超声和生物发光成像，以跟踪体内细胞，许多这些技术受到限制，例如所需的造影剂含量，放射性示踪剂的毒性作用，组织的光衰减和反向散射。随着纳米技术的出现，纳米探针正在引领电荷克服这些局限性。特别是，单壁纳米管（SWNT）已显示被细胞吸收，因此是用于细胞成像的有效纳米探针。因此，这项研究的主要目的是采用含有SWNT纳米探针的间充质干细胞（MSC）来成像细胞在3D支架中的分布，以修复软骨。为此，在细胞培养基（αMEM，10％FBS，1％青霉素/链霉素）中，在32μg/ ml SWNT存在下将MSC培养24小时。确认细胞活力后，将含有SWNT的MSC封装在透明质酸凝胶中，并加载到聚乳酸聚己内酯支架上。在完全的软骨形成培养基中培养28天后，每2天更换一次培养基，通过糖胺聚糖的存在可以确认软骨形成。此外，使用光热光学相干断层扫描（PT-OCT），可以看到细胞以高分辨率分布在支架上。总之，这些数据表明，含有SWNT纳米探针与PT-OCT结合的MSC为体外干细胞跟踪评估播种支架和体内确定生物分布提供了令人兴奋的机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号