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Photo-switchable quantum dots based on reversible FRET

机译：基于可逆FRET的光开关量子点

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摘要

Super-resolution fluorescence microscopy is anticipated to be a powerful tool in observing biological structures and processes smaller than the diffraction limit of light microscopy (～200nm). Yet, many super-resolution techniques (STORM/PALM, STED) employ photo-switchable fluorescent probes (i.e., dyes and fluorescent proteins) that are limited in brightness and stability, reducing potential image resolution. Here, we describe photo-switchable quantum dots (QDs) with enhanced brightness and stability, and excellent optical properties, including narrow emission spectra and broad excitation spectra, compared to fluorescent dyes. These QDs are composed of one green QD, one gold nanoparticle (AuNP), and complimentary single stranded DNA (ssDNA) modified with photo-sensitive azobenzene groups bound to each of the particles. Because of the azobenzene photosensitive property, the ssDNA strands hybridize when excited with visible light, yielding a QD-AuNP conjugate in which QD fluorescence is quenched through Forster resonance energy transfer (FRET); and dehybridize under visible light, yielding separate QDs and AuNPs that are free to diffuse from each other. Because FRET is strongly distance dependent (i.e., α 1/r~6, in this case, a few nanometers), QD fluorescence is restored. Moreover, the photo-switchable QD-AuNP conjugate scheme has the potential to be integrated with a DNA nano-machine platform, adding the potential for photo-manipulated functionality. As a preliminary proof of concept, we tethered different nanocomponents, including QD micelle assemblies and AuNPs, to DNA origami structures (hinge and platform shapes) using ssDNA hybridization.
机译：预期超分辨率荧光显微镜将是观察小于光学显微镜的衍射极限（〜200nm）的生物结构和过程的强大工具。然而，许多超分辨率技术（STORM / PALM，STED）采用光开关荧光探针（即染料和荧光蛋白），其亮度和稳定性受到限制，降低了潜在的图像分辨率。在这里，我们描述了与荧光染料相比具有更高的亮度和稳定性以及出色的光学特性（包括窄发射光谱和宽激发光谱）的光开关量子点（QD）。这些量子点由一个绿色量子点，一个金纳米粒子（AuNP）和互补的单链DNA（ssDNA）组成，这些单链DNA修饰有与每个粒子结合的光敏偶氮苯基。由于偶氮苯具有光敏特性，因此，ssDNA链在可见光激发下发生杂交，从而产生QD-AuNP共轭物，其中QD荧光通过Forster共振能量转移（FRET）猝灭。并在可见光下进行去杂化，产生彼此自由扩散的独立QD和AuNP。因为FRET与距离密切相关（即α1 / r〜6，在这种情况下为几纳米），所以恢复了QD荧光。此外，可光开关的QD-AuNP共轭方案具有与DNA纳米机器平台集成的潜力，从而增加了光操纵功能的潜力。作为概念的初步证明，我们使用ssDNA杂交将不同的纳米成分（包括QD胶束组件和AuNP）束缚到了DNA折纸结构（铰链和平台形状）上。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    Qirui Fan; Gauri Nabar; Carl Miller; Carlos Castro; Jessica Winter;

  • 作者单位

    William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus OH 43210;

    William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus OH 43210;

    Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus OH 43210;

    Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus OH 43210;

    William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus OH 43210,Department of Biomedical Engineering, The Ohio State University, Columbus OH 43210;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Quantum dots; gold nanoparticles; FRET; photo-switchable; azobenzene DNA; DNA origami;

    机译：量子点;金纳米粒子烦恼;光开关偶氮苯DNA; DNA折纸;

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