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Towards a versatile technique for tracking nanoparticle-mucus interaction: a step on the road

机译：迈向跟踪纳米粒子-粘液相互作用的通用技术：迈出的一步

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Respiratory mucus is one of the main barriers for nanoparticle-based pulmonary delivery systems. This holds true especially for lung diseases like cystic fibrosis, where a very tenacious thick mucus layer hinders particle diffusion to the lung epithelium or the target area. Typically, mean square displacement of particles is used for mobility evaluation. In contrast, our objective is to develop a feasible technique to track directed particle penetration as a prerequisite for efficient pulmonary nanotherapy. Therefore, particle diffusion in artificial mucus was monitored based on confocal laser scanning microscopy (CLSM) and particle-mucus interaction was observed. As pharmaceutical relevant and benign materials, solid lipid nanoparticles (SLNs) were prepared by hot-melt emulsification using glyceryl behenate and different stabilizing agents such as poloxamer-407, tween-80, and polyvinyl alcohol (PVA). The diffusion of labeled SLNs in stained artificial sputum representing CF-patient sputum was verified by 3D time laps imaging. Thus, the effect of coating, particle size and mucus viscosity on nanoparticle diffusion was studied. Using image analysis software "Image J", the total fluorescent signal after 30 min in case of poloxamer-coated SLNs was 5 and 100 folds higher than tween- and PVA-coated SLNs, respectively. Nevertheless, increasing mucus viscosity reduced the diffusion of tween-coated SLNs by a factor of 10. Studying particle-mucus interaction by CLSM can be considered a promising and versatile technique.
机译：呼吸粘液是基于纳米颗粒的肺部输送系统的主要障碍之一。这对于像囊性纤维化这样的肺部疾病尤其如此，其中非常顽固的粘液层很厚，阻碍了颗粒扩散到肺上皮或目标区域。通常，颗粒的均方位移用于迁移率评估。相比之下，我们的目标是开发一种可行的技术来跟踪定向的颗粒穿透，将其作为有效的肺部纳米疗法的先决条件。因此，基于共聚焦激光扫描显微镜（CLSM）监测了颗粒在人工黏液中的扩散，并观察到了黏液之间的相互作用。作为药物的相关和良性材料，使用山be酸甘油酯和不同的稳定剂（例如poloxamer-407，tween-80和聚乙烯醇（PVA））通过热熔乳化制备了固态脂质纳米颗粒（SLN）。通过3D时间圈成像，可以验证标记的SLNs在代表CF患者痰液的染色人工痰液中的扩散。因此，研究了包衣，粒径和粘液粘度对纳米粒子扩散的影响。使用图像分析软件“ Image J”，如果涂有泊洛沙姆的SLN，则30分钟后的总荧光信号分别比涂有吐温和PVA的SLN高5倍和100倍。但是，增加粘液粘度会使涂有Tween的SLN的扩散减少10倍。通过CLSM研究颗粒-粘液相互作用可认为是一种有前途且用途广泛的技术。

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