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首页> 外文会议>Nanotechnology VIII >Production of silver nanoparticles by the diatom Phaeodactylum tricornutum
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Production of silver nanoparticles by the diatom Phaeodactylum tricornutum

机译：硅藻Phaeodactylum tricornutum生产银纳米颗粒

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Diatoms (Bacillariophyceae) are the most species-rich group of algae, they are single-celled characterized by a silicified cell wall called a frustule. Diatoms are diverse in shape with many distinct features like raphe and fultoportulae. The diatom cell wall morphology and its hierarchy structure make it a unique unicellular organism for nanotechnology research and applications. Diatom cells are a promising system for green synthesis of nanomaterials like metallic nanoparticles (NPs), nanostructured polymers and other nanomaterials. The production of NPs is achieved today by using methods like attrition or pyrolysis. The cost and the toxic substances often used in these common methods of NPs synthesis limit their applications. Therefore, NPs biosynthesis by diatom cultures, which can be done at ambient CO_2 concentrations, temperature and pressure, offers a sustainable alternative solution. In this work, we examined the formation of silver NPs (AgNPs) by the diatom Phaeodactylum tricornutum cultivated at 25℃ for a period of 8 days. Using this approach, diatom cultures were either grown throughout the duration of the experiment in an artificial seawater (ASW)-f/2 medium enriched with 1 ppm Ag~+ or grown in an ASW-f/2 medium where similar silver ion concentrations were added on experimental day 4. We found that 1 ppm Ag~+ reduces the P. tricornutum growth by up to 50% as compared with the control. Moreover, scanning electron microscopy (SEM) in combination with Energy-Dispersive X-ray (EDX) showed the presence of AgNPs nanoparticles with different sizes and chemical composition associated with the diatom frustules and extracellular polymeric substances.
机译：硅藻（芽孢杆菌科）是藻类中物种最多的一组，它们是单细胞的，其特征是硅化的细胞壁称为截头壳。硅藻的形状各不相同，具有许多独特的特征，例如缝线和fulportportulae。硅藻的细胞壁形态及其层次结构使其成为用于纳米技术研究和应用的独特单细胞生物。硅藻细胞是用于绿色合成纳米材料（如金属纳米颗粒（NPs），纳米结构聚合物和其他纳米材料）的有前途的系统。如今，NP的生产是通过使用损耗或热解等方法实现的。这些常见的NP合成方法中经常使用的成本和有毒物质限制了它们的应用。因此，可以在环境CO_2浓度，温度和压力下进行的硅藻培养合成NPs提供了一种可持续的替代解决方案。在这项工作中，我们研究了在25℃下培养8天的硅藻Phaeodactylum tricornutum的银NPs（AgNPs）的形成。使用这种方法，在整个实验期间，硅藻培养物要么在富含1 ppm Ag〜+的人工海水（ASW）-f / 2培养基中生长，要么在其中银离子浓度相似的ASW-f / 2培养基中生长。在实验第4天添加。我们发现，与对照相比，1 ppm Ag〜+降低了角果疟原虫的生长高达50％。此外，扫描电子显微镜（SEM）结合能量色散X射线（EDX）显示存在与硅藻壳和细胞外聚合物质相关的具有不同大小和化学组成的AgNPs纳米粒子。

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