Novel in vitro transcription assay indicates that the accd nep promoter is contained in a 19 bp fragment

机译：新型体外转录检测表明accd nep启动子包含在19 bp片段中

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In order to characterize promoters for the nuclear-encoded plastid RNA polymerase (NEP), we developed a novel in vitro transcription system. The assay is based on extracts from plants lacking the plastid-encoded RNA polymerase (PEP) activity due to targeted deletion of the PEP #alpha# subunit gene. We report here the characterization of PaccD-129, the promoter for accD, encoding a subunit of the acetyl-CoA carboxylase. Dissection of a approx0.8 kb fragment determined that the accD promoter is contained within a 19-bp fragment (-17/+2).

机译：为了表征启动子的核编码质体RNA聚合酶（NEP），我们开发了一种新型的体外转录系统。该测定基于植物的提取物，这些提取物由于PEP＃alpha＃亚基基因的靶向缺失而缺乏质体编码的RNA聚合酶（PEP）活性。我们在这里报告accD的启动子PaccD-129的特征，编码乙酰辅酶A羧化酶的一个亚基。解剖约0.8 kb的片段可确定accD启动子包含在19 bp片段（-17 / + 2）中。

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《NATO Advanced Research Workshop on The Chloroplast: From Molecular Biology to Biotechnology Kolymbari-Chania, Crete, Greece 10-15 August 1998》|1998年|p.79-84|共6页
会议地点 Kolymbari-Chania(GR)
作者
K. Liere; P. Maliga;
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正文语种 eng
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2. Optimization of a Versatile in Vitro Transcription Assay for the Expression of Multiple Start Site TATA-less Promoters [J] . Yixin Lin, Tan A.Ince, Kathleen W.Scotto Biochemistry . 2001,第43期

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4. Novel in vitro transcription assay indicates that the accd nep promoter is contained in a 19 bp fragment [C] . K. Liere, P. Maliga NATO advanced research workshop on the chloroplast: From molecular biology to biotechnology . 1999

机译：新型体外转录测定表明ACCD NEP启动子包含在19bp片段中
5. Transcriptional repression of the mouse mammary tumor virus promoter in vitro. [D] . Kang, Changjoong. 1994

机译：小鼠乳腺肿瘤病毒启动子的体外转录抑制。
6. Effect of the 21-bp repeat upstream element on in vitro transcription from the early and late SV40 promoters. [O] . M Vigneron, H A Barrera-Saldana, D Baty, 1984

机译：21 bp重复上游元件对早期和晚期SV40启动子体外转录的影响。
7. Possible Involvement of the 5′-Flanking Region and the 5′UTR of Plastid accD Gene in NEP-Dependent Transcription [O] . Plant Cell Physiol, Norihiro Hirata, Daizou Yonekura, 2016

机译：可能参与5'-侧翼区和质体accD基因的5'UTR在NEp依赖性转录中的作用

Novel in vitro transcription assay indicates that the accd nep promoter is contained in a 19 bp fragment

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