首页> 外文会议>NATO Advanced Research Workshop on The Chloroplast: From Molecular Biology to Biotechnology Kolymbari-Chania, Crete, Greece 10-15 August 1998 >Effects of nuclear y mutations on expression of plastid genes required for light-independent chlorophyll formation in chlamydomonas
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Effects of nuclear y mutations on expression of plastid genes required for light-independent chlorophyll formation in chlamydomonas

机译:核Y突变对衣藻光依赖性叶绿素形成所需质体基因表达的影响

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摘要

During evolution two distinct mechanisms were established for the reduction of protochlorophyllide (pchlide) to chlorophyllide (chlide), a key step in the chlorophyll biosynthesis pathway. One mechanism, catalyzed by the enzyme NADPH: protochloro-phyllide oxidoreductase (POR), is completely dependent upon light for its activity. Light-dependent POR is present in cyanobacteria, green algae, most non-vascular and vascular plants, and is the only mechanism used for chlorophyll formation in angio-sperms. The second mechanism, present in anoxygenic photosynthetic bacteria as well as cyanobacteria, non-vascular plants, ferns and gymnosperms, is capable of reducing pchlide to chlide in a light-independent manner. All organisms containing this mechanism for pchlide reduction are capable of chlorophyll formation in the dark. While a significant amount of information is now available on the regulation of POR biosynthesis and activity, little is known about the enzyme mediating light-independent pchlide reduction (designated LIPOR), the factors that regulate its formation, and its requirements for catalytic function.
机译:在进化过程中,建立了两种不同的机制将原叶绿素(pchlide)还原为叶绿素(chlide),这是叶绿素生物合成途径中的关键步骤。 NADPH酶催化的一种机理是:原氯叶绿素氧化还原酶(POR),其活性完全依赖于光。光依赖性POR存在于蓝细菌,绿藻,大多数非维管植物和维管植物中,并且是在精子中形成叶绿素的唯一机制。存在于产氧的光合细菌以及蓝细菌,非维管植物,蕨类植物和裸子植物中的第二种机制能够以不依赖于光的方式将p变为。包含这种减少酰氯的机制的所有生物都能够在黑暗中形成叶绿素。尽管现在可以获得有关POR生物合成和活性调节的大量信息,但对介导光不依赖于光的酰化物还原的酶(称为LIPOR),调节其形成的因素及其对催化功能的要求知之甚少。

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