• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>NATO Advanced Research Workshop on The Chloroplast: From Molecular Biology to Biotechnology Kolymbari-Chania, Crete, Greece 10-15 August 1998 >Proteolytic mechanism in lhcii stabilization
【24h】

Proteolytic mechanism in lhcii stabilization

机译：蛋白水解稳定机制

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

It is now generally accepted that the complicated mechanism regulating Photosynthetic unit (PSU) synthesis/assembly/turnover, in addition to the well known control at the transcriptional and translational level, also involves stringent control at the post-translational level. This became evident to us when working with etiolated plants exposed either to Intermittent light-dark cycles (ImL) (which form only 1/7th of the Chl in plants exposed to continuous light) or first to continuous light and then transferred to the dark. It was then found that (a) the LHCII, which is transcibed and translated in ImL plants, can not be stabilized in their thylakoids, unless the dark phase in the light-dark cycles is shortened; (b) the LHCII preaccumulated in plants exposed to continuous light (CL) is degraded upon transfer of plants to the dark and its Chla is used for the assembly and stabilization of new PSU cores only when preexposure to CL is brief; (c) new LHCII is not stabilized in plants transferred to CL following prolonged preexposure to ImL and no new Chl is formed although the plants are deficient in Chl and LHCII. These results pointed to the involvement on one hand of Chl in stabilizing the newly formed Reaction center and LHC proteins, but also to the involvement of a proteolytic system that could degrade LHC proteins when not rescued by Chl binding. Today an astonishingly large number of chloroplast proteases (stromal, lumenal, thylakoidal) are being characterized and their role in the regulation of PSU assembly and turnover is questioned in many labs. Here we describe our recent findings on the thylakoid-bound protease involved in LHCII stabilization, its developmental regulation and the attempts made for its isolation.
机译：现在已经普遍接受的是，除了众所周知的在转录和翻译水平的控制外，调节光合作用单元（PSU）合成/组装/转换的复杂机制还涉及在翻译后水平的严格控制。对于处理暴露于间歇性明暗循环（ImL）（在连续光照下仅占Chl的1/7的植物）或先暴露于连续光照然后转移到黑暗中的黄化植物而言，这一点对我们而言变得显而易见。然后发现：（a）在ImL植物中被转录和翻译的LHCII不能在其类囊体中稳定，除非缩短了明暗循环中的暗相； （b）在植物转移到黑暗中时，预先暴露在连续光（CL）中的植物中的LHCII会降解，仅当短暂暴露于CL中时，其Chla才可用于组装和稳定新的PSU芯； （c）在长时间暴露于1mL后转移到CL的植物中，新的LHCII不稳定，尽管植物缺乏Chl和LHCII，也没有形成新的Chl。这些结果表明一方面涉及Ch1稳定新形成的反应中心和LHC蛋白，但是也涉及蛋白水解系统的参与，该系统在不通过Ch1结合拯救时可以降解LHC蛋白。如今，令人惊讶的是，大量的叶绿体蛋白酶（基质，内腔，类囊体）已被表征，并且它们在调节PSU组装和周转中的作用受到许多实验室的质疑。在这里，我们描述了我们在与LHCII稳定有关的类囊体结合蛋白酶方面的最新发现，其发育调控以及对其分离的尝试。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号