• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>NATO Advanced Research Workshop on The Chloroplast: From Molecular Biology to Biotechnology Kolymbari-Chania, Crete, Greece 10-15 August 1998 >Characterization of the plastid import reaction of the pea nadph: protochlorophyllide oxidoreductase (POR)
【24h】

Characterization of the plastid import reaction of the pea nadph: protochlorophyllide oxidoreductase (POR)

机译：豌豆幼体的质体输入反应的特征：原绿叶素氧化还原酶（POR）

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

NADPH: protochlorophyllide (POR) is a vital enzyme in the biosynthesis of chlorophyl where it catalyzes the reduction of Pchlide into Chlide in a light-dependent manner. POR is nucleus-encoded and imported into the plastids where it is found at the inner membranes. Together with its substrate and the co-factor NADPH it forms a ternary complex which is needed for catalytical activity. The anomaly of a decreasing POR level during active chlorophyll synthesis was cleared with the discovery of two different POR proteins, POR-A and POR-B, in barley and Arabidopsis thaliana. During greening, POR-A is negatively regulated by light both at transcriptional and proteolytical levels. In addition, the import of POR-A, but not POR-B, has been suggested to require Pchlide in order to be translocated into the plastid. In this respect, POR-A differs from other known nucleus-encoded plastid proteins, and as it appears, this requirements represents a novel and exclusive import characteristic. In pea, only one POR gene has been found indicating that the situation for the regulation of POR import and accumulation is far from clear. We here present a characterization of the import conditions of the pea POR, including the potentional role of Pchlide in the translocation step.
机译：NADPH：原叶绿素内酯（POR）是叶绿素生物合成中的重要酶，它以光依赖的方式催化Pchlide还原为Chlide。 POR是经过核编码的，然后导入质体中的内膜中。与其底物和辅因子NADPH一起形成催化活性所需的三元复合物。由于在大麦和拟南芥中发现了两种不同的POR蛋白POR-A和POR-B，清除了活跃叶绿素合成过程中POR水平降低的异常现象。在绿化期间，POR-A在转录和蛋白水解水平都受到光的负调控。另外，已经提出，进口POR-A而不是POR-B需要Pchlide，以便将其转运到质体中。在这方面，POR-A与其他已知的经核编码的质体蛋白不同，而且看来，这一要求代表了一种新颖且排他的进口特征。在豌豆中，仅发现了一个POR基因，表明调节POR进口和积累的情况尚不清楚。在这里，我们对豌豆POR的进口条件进行了表征，包括Pchlide在易位步骤中的潜在作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号