Towards the 3D structure of 5S rRNA

机译：迈向5S rRNA的3D结构

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The ribosomal 5S RNA is approximately 120 nucleotides long and is an integral part of the large ribosomal subunit. Several parts of the 5S rRNA interact specifically with several ribosomal proteins. Nevertheless its precise role in protein synthesis remains unclear. It is clear that reconstituted 50S ribosomal subunits, lacking the 5S rRNA, are inactive in proteinbiosynthesis. Structural studies will support a more detailed understanding of the 5S rRNA function. Our extensive attempts to crystallise ribosomal 5S RNA have led to crystals, which diffract to about 7.5A. For the investigation of some special features like GU basepairs and hairpin-loops in the ribosomal 5S RNA from Thermus flavus we have divided the 5S rRNA into five domains (A to E) and have solved three crystal structures (Figure 1): Helix I of domain A has been determined at 2.4A. Helix V of domain E without the terminal hairpin-loop has been determined at 1.6A and Helix V of domain E including the terminal hairpin-loop has been determined at 3.0A. Furthermore it was possible to observe a general mode of intermolecular interaction, which occur only in RNA structures and it was possible to demonstrate the structural importance of water molecules for the stability of RNA-molecules.

机译：核糖体5S RNA长约120个核苷酸，是大核糖体亚基的组成部分。 5S rRNA的几个部分与几种核糖体蛋白特异性相互作用。然而，其在蛋白质合成中的确切作用仍不清楚。显然，缺乏5S rRNA的重组50S核糖体亚基在蛋白质生物合成中没有活性。结构研究将支持对5S rRNA功能的更详细的了解。我们广泛尝试使核糖体5S RNA结晶，导致晶体衍射至约7.5A。为了研究Thermus flavus核糖体5S RNA中GU碱基对和发夹环的某些特殊特征，我们将5S rRNA分为五个结构域（A到E），并解析了三个晶体结构（图1）：域A已确定为2.4A。没有末端发夹环的结构域E的螺旋V已确定为1.6A，包括末端发夹环的结构域E的螺旋V已确定为3.0A。此外，有可能观察到仅在RNA结构中发生的分子间相互作用的一般模式，并且有可能证明水分子对RNA分子的稳定性的结构重要性。

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《NATO Advanced Research Workshop on RNA Biochemistry and Biotechnology Poznan, Poland October 10-17, 1998》|1998年|p.63-71|共9页
会议地点 Poznan(PL)
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M. Perbandt; S. Lorenz; M. Vallazza; V.A. ERdmann; C. Betzel;
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2. Chromosomal mapping of 5S and 18S-5.8S-25S rRNA genes in Saccharina japonica(Phaeophyceae)as visualized by dual-color fluorescence in situ hybridization [J] . Yu LIU, Pengfei LIU, Yanhui BI, 海洋湖沼学报（英文） . 2021,第002期
3. Nucleotide sequences of the spacer-1, spacer-2 and trailer regions of the rrn operons and secondary structures of precursor 23S rRNAs and precursor 5S rRNAs of slow-growing mycobacteria [J] . Yuan-en Ji, Karen E. Kempsell, M. Joseph Colston, Microbiology . 1995,第2期

机译：间隔物-1，间隔件-2和RRN操纵子的拖车区域的核苷酸序列和第23s的前体23s rRNA和前体5s的二次结构，并且生长缓慢的分枝杆菌
4. Nucleotide sequences of the spacer-1, spacer-2 and trailer regions of the rrn operons and secondary structures of precursor 23S rRNAs and precursor 5S rRNAs of slow-growing mycobacteria [J] . YUAN-EN JI, KAREN E. KEMPSELL, M. JOSEPH COLSTON, Microbiology . 1994,第7期

机译：间隔物-1，间隔件-2和RRN操纵子的拖车区域的核苷酸序列和第23s的前体23s rRNA和前体5s的二次结构，并且生长缓慢的分枝杆菌
5. An E-coli 5S rRNA deletion mutant useful for the study of 5S rRNAstructure/function relationships [J] . Ammons D, Rampersad J Current Microbiology: An International Journal . 2001,第2期

机译：大肠杆菌5S rRNA缺失突变体，可用于研究5S rRNA结构/功能关系
6. Towards the 3D structure of 5S rRNA [C] . M. Perbandt, S. Lorenz, M. Vallazza, NATO advanced research workshop on RNA biochemistry and biotechnology . 1999

机译：朝向5S rRNA的3D结构
7. Study of common Vibrio 5S ribosomal RNA sequence variants and development of a new methodology to identify the phenotypes of Vibrio proteolyticus 5S rRNA variants. [D] . Zhang, Zhengdong. 2002

机译：常见的弧菌5S核糖体RNA序列变异体的研究和一种新方法的开发，以鉴定蛋白水解弧菌5S rRNA变异体的表型。
8. Heterogeneity in the 3-portion of Papilionaceae 5S rRNAs. The primary structure of alfalfa 5S rRNA. [O] . M Z Barciszewska, M Nalaskowska, T D Mashkova, 1987

机译：凤蝶科5S rRNA 3部分的异质性。苜蓿5S rRNA的一级结构。
9. Heterogeneity in the 3'-portion of Papilionaceae 5S rRNAs. The primary structure of alfalfa 5S rRNA. [O] . Barciszewska, M Z, Nalaskowska, M, Mashkova, T D, 1987

机译：凤蝶科5S rRNA 3'部分的异质性。苜蓿5S rRNA的一级结构。

Towards the 3D structure of 5S rRNA

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