• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>NATO Advanced Study Institute on Polymer Based Systems on Tissue Engineering, Replacement and Regeneration Oct 15-25, 2001 Alvor, Algarve, Portugal >CULTURED BONE ON BIOMATERIAL SUBSTRATES A Tissue Engineering Approach to Treat Bone Defects
【24h】

CULTURED BONE ON BIOMATERIAL SUBSTRATES A Tissue Engineering Approach to Treat Bone Defects

机译：在生物材料基质上培养骨的组织工程方法来治疗骨缺损

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In the present work, a tissue engineering approach to treat bone defects was investigated. Such strategy was based on the use of patient own cultured bone marrow stromal cells (BMSCs) in association with biomaterials to produce autologous living bone equivalents. When engineering such implants, three main factors had to be taken into account: (ⅰ) the cells, (ⅱ) the culture technology and (ⅲ) the biomaterial scaffolds. The capacity of BMSCs to proliferate, differentiate along the osteogenic lineage and form a bone like tissue was demonstrated in various in vitro assays making use of biochemical, immunological, microscopic and gene expression techniques. The ability of the cells to produce bone in vivo was established using an ectopic (extra osseous) implantation model. Results indicated that BMSC cultures were composed of a heterogeneous population containing a subpopulation of cells with high proliferative capacity and with potential to differentiate into bone forming cells. Both the growth and the differentiation pattern of these cells could be manipulated, to a certain degree, through the use of bioactive factors during culture. After implantation, the bone forming capacity of the cultures proved to be related to the amount of early osteoprogenitors and precursors cells that could be induced into starting the osteogenic differentiation process. In bone marrow aspirates, this subpopulation appeared to decrease with donor age and to be strongly dependent on the donor, indicating that the aspiration procedure plays an important role in the obtained bone marrow cell population. In order to evaluate the in vivo bone formatior capacity of BMSC cultures prior to implantation, an experimental method was developed in which the amount of early osteoprogenitors and precursors cells could be quantified. With regard to the technology design, data indicated that the culture of cells on the biomaterial scaffolds prior to implantation resulted in implants with faster in vivo bone forming ability as compared to scaffolds implanted shortly after cell seeding. In addition, two biodegradable polymeric systems were proposed as scaffolds to be used in the described bone engineering approach after evaluating their ability to support bone marrow cell growth, differentiation and in vivo bone formation. In summary, although the complete knowledge of the factors controlling BMSC growth and osteogenic differentiation still needs to be further expanded, the obtained results suggest that the bone tissue engineering approach described in this work presents a great potential for the repair of bone defects and will become an advantageous alternative to the traditional autologous bone grafting.
机译：在目前的工作中，研究了一种组织工程方法来治疗骨缺损。这种策略是基于将患者自身培养的骨髓基质细胞（BMSC）与生物材料结合使用，以产生自体活骨等效物。在设计此类植入物时，必须考虑三个主要因素：（ⅰ）细胞，（ⅱ）培养技术和（ⅲ）生物材料支架。利用生化，免疫，显微镜和基因表达技术的各种体外试验证明了BMSCs沿成骨细胞系增殖，分化和形成骨样组织的能力。使用异位（骨外）植入模型来建立细胞在体内产生骨骼的能力。结果表明，BMSC培养物由异质群体组成，其中包含具有高增殖能力并具有分化成骨形成细胞潜能的细胞亚群。通过在培养过程中使用生物活性因子，可以在一定程度上控制这些细胞的生长和分化模式。植入后，培养物的骨形成能力证明与可被诱导开始成骨分化过程的早期骨祖细胞和前体细胞的数量有关。在骨髓抽吸物中，该亚群似乎随着供体的年龄而减少，并且强烈依赖于供体，这表明抽吸过程在获得的骨髓细胞群中起着重要的作用。为了评估BMSC培养物在植入前的体内骨形成能力，开发了一种实验方法，其中可以量化早期骨祖细胞和前体细胞的数量。关于技术设计，数据表明，与植入细胞后不久植入的支架相比，植入前在生物材料支架上的细胞培养导致植入物具有更快的体内骨骼形成能力。另外，在评估了它们支持骨髓细胞生长，分化和体内骨骼形成的能力之后，提出了两种可生物降解的聚合物系统作为支架，用于所述的骨工程方法中。综上所述，尽管对控制BMSCs生长和成骨分化的因素的全面了解仍需进一步拓展，但获得的结果表明，这项工作中描述的骨组织工程方法具有修复骨缺损的巨大潜力，并将成为传统自体骨移植的一种有利替代方案。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号