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首页> 外文会议>Ophthalmic technologies XXII >In vivo quantification of microglia dynamics with a scanning laser ophthalmoscope in a mouse model of focal laser injury
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In vivo quantification of microglia dynamics with a scanning laser ophthalmoscope in a mouse model of focal laser injury

机译：在聚焦激光损伤的小鼠模型中使用扫描激光检眼镜在体内量化小胶质细胞动力学

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摘要

Microglia are the resident immune cells of the central nervous system and play a crucial role in maintaining neuronal health and function. Their dynamic behavior, that is, the constant extension and retraction of microglia processes, is thought to be critical for communication between microglia and their cellular neighbors, such as neurons, astrocytes and vascular endothelial cells.Here, we investigated the morphology and dynamics of retinal microglia in vivo under normal conditions and in response to focal laser injury of blood vessel endothelial wall, using a scanning laser ophthalmoscope (SLO) designed specifically for imaging the retina of live mice. The multichannel confocal imaging system allows retinal microstructure, such as the processes of microglia and retinal vasculature, to be visualized simultaneously. In order to generate focal laser injury, a photocoagulator based on a continuous wave (cw) laser was coupled into the SLO. An acousto-optic modulator chopped pulses from the cw laser. A tip-tilt-scanner was used to direct the laser beam into a blood vessel of interest under SLO image guidance. Mild coagulation was produced using millisecond-long pulses.Microglia react dynamically to focal laser injury of blood vessel endothelial walls. Under normal conditions, microglia somas remain stationary and the processes probe a territory of their immediate environment. In response to local injury, process movement velocity approximately doubles within minutes after injury. Moreover, the previously unpolarized process movement assumes a distinct directionality towards the injury site, indicating signaling between the injured tissue and the microglia. In vivo retinal imaging is a powerful tool for understanding the dynamic behavior of retinal cells.
机译：小胶质细胞是中枢神经系统的固有免疫细胞，在维持神经元健康和功能中起着至关重要的作用。它们的动态行为，即小胶质细胞过程的持续扩展和收缩，被认为对于小胶质细胞及其邻近细胞（例如神经元，星形胶质细胞和血管内皮细胞）之间的通讯至关重要。在这里，我们研究了视网膜的形态和动力学使用专门设计用于对活小鼠视网膜成像的扫描激光检眼镜（SLO），在正常条件下并响应血管内皮壁的聚焦激光损伤而在体内产生小胶质细胞。多通道共聚焦成像系统可以同时显示视网膜的微结构，例如小胶质细胞和视网膜脉管系统的过程。为了产生聚焦激光损伤，将基于连续波（cw）激光的光凝器耦合到SLO中。声光调制器将连续波激光器的脉冲斩波。在SLO图像引导下，使用倾斜扫描仪将激光束引导到感兴趣的血管中。使用毫秒级脉冲产生轻度凝结。小胶质细胞对血管内皮壁的聚焦激光损伤有动态反应。在正常情况下，小胶质细胞体保持静止，并且过程探测其周围环境的区域。为响应局部伤害，伤害后几分钟内过程移动速度大约增加一倍。此外，先前未极化的过程运动呈现出指向损伤部位的明显方向性，表明在受伤的组织和小胶质细胞之间发出信号。体内视网膜成像是了解视网膜细胞动态行为的有力工具。

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  • 来源
    《Ophthalmic technologies XXII》|2012年|p.820907.1-820907.9|共9页
  • 会议地点 San Francisco CA(US)
  • 作者

    Clemens Alt; Charles P. Lin;

  • 作者单位

    Advanced Microscopy Program, Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School;

    Advanced Microscopy Program, Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 眼科学;
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