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首页> 外文会议>Ophthalmic technologies XXII >SPECTROSCOPIC MEASUREMENTS DURING THE CORNEAL COLLAGEN CROSS-LINKING PROCEDURE FOR IN VITRO HUMAN CORNEAS
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SPECTROSCOPIC MEASUREMENTS DURING THE CORNEAL COLLAGEN CROSS-LINKING PROCEDURE FOR IN VITRO HUMAN CORNEAS

机译：角膜胶原交联过程中体外人角膜的光谱测量

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摘要

The transmittance of UVA light through the human preserved cornea of over 400um thickness during the corneal collagen cross-linking procedure has been measured spectroscopically. The 25 corneas, (average thickness of 570 μm), preserved in OptisolGS, were washed with saline, desepithelization was performed, and the cornea was laid on the lid of a Chiron Ophthalmics corneal storage chamber. A UV-VIS optical fiber was positioned at the crystalline position (10mm after the endothelium) and fixed in a 3mm hole of the chamber and then connected to a spectrophotometer to detect the amount of delivered UVA light on the endothelium. Current procedure protocol was performed, i.e., one drop of riboflavin 0.1%, 400 mOsm, was applied on the naked cornea, every 5 minutes (total of 12 drops). The UV irradiation (365±5 nm, 3mW/cm~2, 1.51 mW, 5.405 J/cm~2) was performed after 30 min of instillation for an additional 30 min. The average transmittance of the desepithelized cornea without Riboflavin at the crystalline position is 65.8%'; after the 1st drop of Riboflavin, transmittance is 51.4%; after 2nd drop, 46.1%; after 3rd drop, 41.9% ; after 4th drop, 38.7%; after 5th drop, 35.9%; after 6th drop 33.6% ; after 7th drop, 31.0%; after 8* drop; 28.8%; after 9* drop, 27.2%; after 10h drop, 25.4%; after 11th drop, 23.9%; and finally after 12* drop, 22.5%. The average transmittance in terms of energy during the 30 min irradiation procedure fluctuated from 0.930 to 0.675mW/cm2.
机译：已经通过光谱法测量了在角膜胶原交联过程中UVA光通过人类保存的超过400um厚度的角膜的透射率。将保存在OptisolGS中的25个角膜（平均厚度为570μm）用盐水洗涤，进行脱皮，然后将角膜放在Chiron Ophthalmics角膜储藏室的盖子上。将UV-VIS光纤放置在晶体位置（内皮后10毫米）并固定在腔室的3毫米孔中，然后将其连接到分光光度计上，以检测在内皮上传递的UVA光量。进行当前程序操作，即每5分钟将一滴0.1％的核黄素400mOsm滴在裸角膜上（总共12滴）。滴加30分钟后再进行30分钟的紫外线照射（365±5 nm，3mW / cm〜2，1.51 mW，5.405 J / cm〜2）。在结晶位置不含核黄素的去皮上角膜的平均透射率为65.8％'；核黄素1滴后，透射率为51.4％；第二次下跌后，占46.1％；第三跌后，为41.9％;排名第4后下降38.7％；第5跌后，涨幅为35.9％；第六次下降33.6％;排名第7后下降31.0％； 8 *下降后； 28.8％；下降9 *后，27.2％;下降10h后，占25.4％；排名第11后下降23.9％；最后下跌12 *后，跌幅为22.5％。在30分钟的照射过程中，平均能量透射率从0.930波动至0.675mW / cm2。

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