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首页> 外文会议>Optical biopsy XV: Toward real-time spectroscopic imaging and diagnosis >In vivo measurement of the tissue oxygenation by time-resolved luminescence spectroscopy of protoporphyrin IX: Strategies to minimize artefacts associated with photoproducts
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In vivo measurement of the tissue oxygenation by time-resolved luminescence spectroscopy of protoporphyrin IX: Strategies to minimize artefacts associated with photoproducts

机译：通过时间分辨发光光谱对原卟啉IX进行体内氧合作用的体内测量：最大限度地减少与光产品有关的伪影的策略

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The determination of the oxygen partial pressure (pO_2) in real time in living biological tissues is of high interest for numerous therapeutics, including photodynamic therapy (PDT) and radiotherapy. The minimally invasive and real-time measurement of the pO_2 also enables to obtain interesting fundamental information regarding the metabolic activities in cells and tissues. The development of time-resolved luminescence measurement (TRLM) methods combined with the availability of new oxygen-sensitive molecular probes is at the origin of the significant progress that have been achieved during these past decades to measure the pO_2 in living organisms. These probes include porphyrins, such as aminolevulinic acid-induced protoporphyrin IX (PPIX), which is an approved photosensitizer. Using the photosensitizer to probe the pO_2 is of high interest in PDT since the level of oxygen is measured at the precise location where the phototoxic mechanisms take place. However, PPIX has drawbacks to measure the pO_2 by TRLM, including its significant photobleaching. Since the PPIX excitation during pO_2 measurements leads to the generation of its photoproducts, we studied the impact of their luminescence on the measurement of the PPIX triplet state lifetime in solution and in vivo on the Chick's Chorioallantoic Membrane (CAM) model. We performed this study under various oxygen conditions. Our results indicate that perturbations induced by these photoproducts can be avoided if the PPIX luminescence is detected between 620 and 640 nm, or if PPIX is excited at 405 nm with light doses < 1 J/cm~2.
机译：实时确定活生物组织中的氧分压（pO_2）对于包括光动力疗法（PDT）和放射疗法在内的许多治疗方法都非常重要。 pO_2的微创和实时测量还可以获取有关细胞和组织中代谢活动的有趣基础信息。时间分辨发光测量（TRLM）方法的发展与新的对氧敏感的分子探针的可用性相结合，是过去几十年来在测量活生物体中pO_2方面取得的重大进展的起点。这些探针包括卟啉，例如氨基乙酰丙酸诱导的原卟啉IX（PPIX），这是一种认可的光敏剂。使用光敏剂探测pO_2在PDT中引起了极大的兴趣，因为在发生光毒性机理的确切位置测量了氧气的水平。但是，PPIX的缺点是无法通过TRLM测量pO_2，包括其显着的光致漂白。由于在pO_2测量过程中PPIX激发导致其光产物的产生，因此我们研究了它们的发光对溶液和体内的PPIX三重态寿命的测量对小鸡Chorioallantoic膜（CAM）模型的影响。我们在各种氧气条件下进行了这项研究。我们的结果表明，如果在620至640 nm之间检测到PPIX发光，或者如果PPIX在405 nm处以小于1 J / cm〜2的光激发，则可以避免由这些光产物引起的扰动。

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