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Optimizing penetration depth, contrast, and resolution in 3D dermatologic OCT

机译:在3D皮肤科OCT中优化穿透深度,对比度和分辨率

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摘要

High speed, three-dimensional optical coherence tomography (3D OCT) at 800nm, 1060nm and 1300nm with approximately 4μm, 7μm and 6μm axial and less than 15μm transverse resolution is demonstrated to investigate the optimum wavelength region for in vivo human skin imaging in terms of contrast, dynamic range and penetration depth. 3D OCT at 1300nm provides deeper penetration, while images obtained at 800nm were better in terms of contrast and speckle noise. 1060nm region was a compromise between 800nm and 1300nm in terms of penetration depth and image contrast. Optimizing sensitivity, penetration and contrast enabled unprecedented visualization of micro-structural morphology underneath the glabrous skin, hairy skin and in scar tissue. Higher contrast obtained at 800 nm appears to be critical in the in vitro tumor study. A multimodal approach combining OCT and PA helped to obtain morphological as well as vascular information from deeper regions of skin.
机译:高速,三维光学相干断层扫描(3D OCT)在800nm,1060nm和1300nm处具有大约4μm,7μm和6μm的轴向分辨率和小于15μm的横向分辨率,被证明可以从以下方面研究人体皮肤成像的最佳波长范围:对比度,动态范围和穿透深度。 1300nm处的3D OCT提供更深的穿透力,而800nm处获得的图像在对比度和斑点噪声方面更好。就穿透深度和图像对比度而言,1060nm区域是800nm和1300nm之间的折衷。优化的灵敏度,穿透力和对比度使无毛皮肤,毛发皮肤和疤痕组织下的微结构形态得以前所未有的可视化。在800 nm处获得更高的对比度似乎在体外肿瘤研究中至关重要。结合了OCT和PA的多模式方法有助于从皮肤深处获得形态和血管信息。

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