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首页> 外文会议>Optical Coherence tomography and coherence domain optical methods in biomedicine XIX >High resolution polarization sensitive OCT for ocular imaging in rodents
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High resolution polarization sensitive OCT for ocular imaging in rodents

机译：高分辨率偏振敏感OCT，用于啮齿动物的眼部成像

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摘要

A new high-resolution polarization sensitive optical coherence tomography system was developed for imaging rodent retina. Various light-tissue interactions such as birefringence and depolarization can change the polarization state of light. In the eye, there are several tissues that have these properties, for example retinal pigment epithelium (depolarization) and sclera (birefringence). These layers play key roles in diseases like age-related macular degeneration or glaucoma. Animal models are an important component for understanding disease pathogenesis. The gold standard for the evaluation of preclinical experiments is histology, which is an invasive and terminal procedure. Since OCT is non-invasive, it has the potential to be an alternative to histology with the benefit of long-term study of the disease progression in the same animal. In this study, a superluminescent diode with spectrum width 100 nm and mean wavelength 840 nm is used as a light source in order to enable high axial resolution. Spectrometers are custom built to enable high imaging speed that allows acquiring 3D data sets with 1024×200×1536 voxels in 3.44 s. From the acquired data, images displaying phase retardation induced by birefringence and orientation of birefringent axis were calculated. In first measurements, we were able to identify the RPE-choroid complex (depolarization effect) and the sclera (strong birefringence) in the retina of Long-Evans and Sprague-Dawley rats. Our preliminary results demonstrate the feasibility of the system for high speed/resolution imaging of the rodent retina. This is useful for longitudinal studies of disease models of retinal disease in rats and mice.
机译：开发了一种新的高分辨率偏振敏感光学相干层析成像系统，用于对啮齿动物视网膜成像。各种光组织相互作用（例如双折射和去偏振）可以改变光的偏振状态。在眼睛中，有几种具有这些特性的组织，例如视网膜色素上皮（去极化）和巩膜（双折射）。这些层在与年龄有关的黄斑变性或青光眼等疾病中起关键作用。动物模型是了解疾病发病机制的重要组成部分。评估临床前实验的金标准是组织学，这是一种侵入性的终末过程。由于OCT是非侵入性的，因此可以长期研究同一只动物的疾病进展，因此有可能替代组织学。在这项研究中，使用光谱宽度为100 nm，平均波长为840 nm的超发光二极管作为光源，以实现较高的轴向分辨率。光谱仪是定制制造的，以实现高成像速度，从而可以在3.44 s内以1024×200×1536体素采集3D数据集。根据获取的数据，计算出显示由双折射引起的相位延迟和双折射轴的取向的图像。在第一次测量中，我们能够确定Long-Evans和Sprague-Dawley大鼠视网膜中的RPE-脉络膜复合物（去极化作用）和巩膜（强双折射）。我们的初步结果证明了该系统对啮齿类动物视网膜进行高速/高分辨率成像的可行性。这对于纵向研究大鼠和小鼠的视网膜疾病的疾病模型很有用。

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  • 会议地点 San Francisco CA(US)
  • 作者

    Stanislava Fialova; Sabine Rauscher; Marion Groeger; Michael Pircher; Christoph K. Hitzenberger; Bernhard Baumann;

  • 作者单位

    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria,Medical Imaging Cluster, Medical University of Vienna, Austria;

    Medical Imaging Cluster, Medical University of Vienna, Austria,Core Facility Imaging, Medical University of Vienna, Austria;

    Medical Imaging Cluster, Medical University of Vienna, Austria,Core Facility Imaging, Medical University of Vienna, Austria;

    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria,Medical Imaging Cluster, Medical University of Vienna, Austria;

    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria,Medical Imaging Cluster, Medical University of Vienna, Austria;

    Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria,Medical Imaging Cluster, Medical University of Vienna, Austria;

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  • 正文语种 eng
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  • 关键词

    optical coherence tomography; polarization sensitive; high resolution; rodents; retina;

    机译：光学相干断层扫描；极化敏感高分辨率;啮齿动物视网膜;

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