• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Optical Coherence tomography and coherence domain optical methods in biomedicine XX >Diffusion-sensitive optical coherence tomography for real-time monitoring of mucus thinning treatments
【24h】

Diffusion-sensitive optical coherence tomography for real-time monitoring of mucus thinning treatments

机译：扩散敏感光学相干断层扫描技术，用于实时监测粘液稀化治疗

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mucus hydration (wt%) has become an increasingly useful metric in real-time assessment of respiratory health in diseases like cystic fibrosis and COPD, with higher wt% indicative of diseased states. However, available in vivo rheological techniques are lacking. Gold nanorods (GNRs) are attractive biological probes whose diffusion through tissue is sensitive to the correlation length of comprising biopolymers. Through employment of dynamic light scattering theory on OCT signals from GNRs, we find that weakly-constrained GNR diffusion predictably decreases with increasing wt% (more disease-like) mucus. Previously, we determined this method is robust against mucus transport on human bronchial epithelial (hBE) air-liquid interface cultures (R~2=0.976). Here we introduce diffusion-sensitive OCT (DS-OCT), where we collect M-mode image ensembles, from which we derive depth- and temporally-resolved GNR diffusion rates. DS-OCT allows for real-time monitoring of changing GNR diffusion as a result of topically applied mucus-thinning agents, enabling monitoring of the dynamics of mucus hydration never before seen. Cultured human airway epithelial cells (Calu-3 cell) with a layer of endogenous mucus were doped with topically deposited GNRs (80×22nm), and subsequently treated with hypertonic saline (HS) or isotonic saline (IS). DS-OCT provided imaging of the mucus thinning response up to a depth of 600μm with 4.65μm resolution, over a total of 8 minutes in increments of ≥3 seconds. For both IS and HS conditions, DS-OCT captured changes in the pattern of mucus hydration over time. DS-OCT opens a new window into understanding mechanisms of mucus thinning during treatment, enabling real-time efficacy feedback needed to optimize and tailor treatments for individual patients.
机译：粘液水合（wt％）已成为实时评估诸如囊性纤维化和COPD等疾病的呼吸健康状况的一项越来越有用的指标，其中wt％较高表示疾病状态。但是，缺乏可用的体内流变技术。金纳米棒（GNR）是有吸引力的生物探针，其在组织中的扩散对所含生物聚合物的相关长度敏感。通过对来自GNRs的OCT信号使用动态光散射理论，我们发现弱约束的GNR扩散可预测地随着wt％（更像疾病样）的粘液增加而减少。先前，我们确定了该方法对于在人支气管上皮（hBE）气液界面培养物中的粘液运输具有鲁棒性（R〜2 = 0.976）。在这里，我们介绍了扩散敏感的OCT（DS-OCT），我们在其中收集M模式图像集合，从中得出深度和时间分辨的GNR扩散速率。 DS-OCT可以实时监测由于局部应用粘液稀释剂而产生的GNR扩散变化，从而可以监测前所未有的粘液水化动力学。将具有内源性粘液层的培养的人气道上皮细胞（Calu-3细胞）掺入局部沉积的GNR（80×22nm），然后用高渗盐水（HS）或等渗盐水（IS）处理。 DS-OCT提供了对黏液稀薄反应的成像，分辨率达4.65μm，深度达600μm，共8分钟，增量≥3秒。对于IS和HS条件，DS-OCT都能捕获粘液水化模式随时间的变化。 DS-OCT为了解治疗期间粘液变薄的机制打开了一个新窗口，从而为优化和定制针对个别患者的治疗提供了实时功效反馈。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号