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Monitoring Biofilm Attachment on Medical Device Surfaces Using Hyperspectral Imaging

机译:使用高光谱成像监控医疗设备表面上的生物膜附着

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Microbial biofilm is a colony of single bacteria cells (planktonic) that attach to surfaces, attract other microorganisms to attach and grow, and together build an extracellular matrix composed of polysaccharides, protein, and DNA. Furthermore, some cells may detach and spread to other surface. Biofilm on medical devices can cause severe infection to all age ranges from infant to adult. Therefore, it is important to detect biofilm in a fast and efficient manner. Hyperspectral Imaging was utilized for distinguishing a wide area of biofilm coverage on various materials and on different textures of stainless steel test coupons. In addition to biofilm coverage, the shear stress of biofilm on attached surfaces is also important. The present study investigates the effect of shear stress on the adhesion of biofilms on common medical device surfaces such as glass, polycarbonate, polytetrafluoroethylene, and stainless steel with different textures. Biofilm was grown using Pseudomonas aeruginosa and growth was monitored after 24 and 48 hours at 37° C. The coupons covered with biofilm were tilted at 45 degrees and 90 degrees for 30 seconds to induce shear stress and Hyperspectral Images were taken. We hypothesize that stronger attachment on rough surface withstands greater shear stress compared to smooth surface. The results have verified our hypothesis with more biofilm persists on brushed surface such as stainless steel than on un-brushed surface like mirror-like or glass.
机译:微生物生物膜是附着在表面的单个细菌细胞(浮游生物)的集落,吸引其他微生物附着并生长,并共同构成由多糖,蛋白质和DNA组成的细胞外基质。此外,某些细胞可能分离并扩散到其他表面。医疗设备上的生物膜可导致从婴儿到成人的所有年龄段的严重感染。因此,以快速有效的方式检测生物膜很重要。高光谱成像用于区分各种材料和不锈钢测试样的不同纹理上的生物膜覆盖范围。除了覆盖生物膜外,附着表面上生物膜的剪切应力也很重要。本研究调查了剪切应力对生物膜在常见医疗器械表面(如玻璃,聚碳酸酯,聚四氟乙烯和具有不同纹理的不锈钢)上的粘附力的影响。使用铜绿假单胞菌(Pseudomonas aeruginosa)生长生物膜,并在37℃下24和48小时后监测生长。将覆盖有生物膜的试样在45度和90度倾斜30秒以引起剪切应力,并拍摄高光谱图像。我们假设与光滑表面相比,在粗糙表面上更牢固的附着可承受更大的剪切应力。结果证实了我们的假设,即在诸如不锈钢之类的拉丝表面上存在的生物膜比在像镜子或玻璃之类的非拉丝表面上存在更多的生物膜。

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