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首页> 外文会议>Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VI; Progress in Biomedical Optics and Imaging; vol.7 no.6 >Cell-based Bio-optical Sensors Using Chalcogenide Fibers
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Cell-based Bio-optical Sensors Using Chalcogenide Fibers

机译：使用硫族化物纤维的基于细胞的生物光学传感器

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An optical bio-sensor is built based on an infrared chalcogenide fiber coated with live cells. The fiber is immersed in an aqueous media appropriate for cell viability. The response of the cells to small quantities of toxicant can be monitored spectroscopically. The properties of chalcogenide fibers used for cell-based biosensors are investigated. The chemical stability of Te-As-Se fibers in aqueous media is shown to depend on the previous storage time of the fiber. Older fibers are shown to generate an oxide layer during extended exposure to air. This layer readily dissolves in aqueous solution and causes the release of As in the cell environment. The release of As during dissolution of the oxide layer is measured with ICP-MS and is shown to be complete after a couple hours. Fresh fibers do not show any detectable oxide layer and show excellent stability in aqueous solution. The surface roughness of old and fresh fibers is investigated with AFM before and after dissolution in aqueous media. Old fibers immersed in solution show sizable roughness due to the oxide surface layer dissolution. Fresh fibers do not show any detectable changes even after extended immersion in aqueous solution. The toxicity of As to various types of vertebrate cells is quantified using a colorimetric assay. Old fibers are shown to be notably toxic due to As released during dissolution. The fiber toxicity is shown to decrease when the fibers are previously washed in solution. The toxicity of the resulting wash water is then shown to increase due to the increase in As concentration.
机译：光学生物传感器是基于涂有活细胞的红外硫族化物纤维构建的。将纤维浸入适合细胞活力的水性介质中。可以通过光谱监测细胞对少量毒物的反应。研究了用于基于细胞的生物传感器的硫族化物纤维的特性。显示Te-As-Se纤维在水性介质中的化学稳定性取决于纤维的先前存储时间。显示较旧的纤维在长时间暴露于空气中会产生氧化层。该层易于溶解在水溶液中，并导致在细胞环境中释放出As。用ICP-MS测定了氧化物层溶解过程中As的释放，并显示在几个小时后已完成。新鲜纤维没有显示任何可检测的氧化物层，并且在水溶液中显示出极好的稳定性。在含水介质中溶解之前和之后，使用AFM研究新旧纤维的表面粗糙度。浸入溶液中的旧纤维由于氧化物表面层溶解而显示出相当大的粗糙度。即使长时间浸入水溶液中，新鲜纤维也不会显示任何可检测到的变化。使用比色测定法定量了As对各种类型的脊椎动物细胞的毒性。由于溶解过程中释放出的砷，表明旧纤维具有明显的毒性。当预先在溶液中洗涤纤维时，表明纤维毒性降低。结果表明，由于砷浓度的增加，所得洗涤水的毒性增加。

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