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首页> 外文会议>Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VI; Progress in Biomedical Optics and Imaging; vol.7 no.6 >Compact fluorescence detection using in-fiber microchannels
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Compact fluorescence detection using in-fiber microchannels

机译：使用光纤微通道的紧凑型荧光检测

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摘要

Applications involving fluorescence detection in point-of-care systems are both interesting and challenging in nature. The applications usually require a simple, compact, robust, highly sensitive yet affordable system. As a result, the system needs to be efficient in fluorescence detection by using practical and easily fabricated, hence inexpensive sensors. In this paper, a fluorescence sensor using an in-fiber microchannel has been developed and tested successfully. A blue LED, multimode PMMA or silica fiber, mini-PMT and fluorescein in PBS pH 7.4 buffer solution were used as the excitation source, light guide, fluorescence detector and sample, respectively. Microfluidic channels of 100μm width and 1cm length were fabricated in the optical fibers using a direct write CO_2 laser system. The channels in the fibers were examined using a SEM and an optical microscope. Experimental results show that the sensor is highly sensitive, being able to detect 0.1 μg/L of fluorescein in the PBS buffer solution, with good signal to noise ratio and the results are reproducible. The data obtained using silica fibers as sensors when compared with the results from PMMA fibers show that the silica fiber sensor has better sensitivity than the PMMA fiber sensor. This could be due to the fouling effect created by the frosty layer at the bottom of the microchannel made within the PMMA fiber. Our future work will integrate the fiber sensor into microfluidic chips for lab-on-a-chip applications.
机译：本质上，涉及即时检测系统中荧光检测的应用既有趣又充满挑战。这些应用程序通常需要一个简单，紧凑，健壮，高度敏感但价格适中的系统。结果，该系统需要通过使用实用且易于制造的廉价传感器来高效地进行荧光检测。在本文中，已成功开发并测试了使用光纤微通道的荧光传感器。 PBS pH 7.4缓冲溶液中的蓝色LED，多模PMMA或石英纤维，mini-PMT和荧光素分别用作激发源，光导，荧光检测器和样品。使用直接写入CO_2激光系统在光纤中制造了宽度为100μm，长度为1cm的微流体通道。使用SEM和光学显微镜检查纤维中的通道。实验结果表明，该传感器具有很高的灵敏度，能够检测PBS缓冲溶液中的荧光素0.1μg/ L，信噪比良好，结果具有可重复性。与来自PMMA纤维的结果相比，使用二氧化硅纤维作为传感器获得的数据表明，二氧化硅纤维传感器具有比PMMA纤维传感器更好的灵敏度。这可能是由于在PMMA纤维内的微通道底部的霜层所产生的结垢效果。我们未来的工作将把光纤传感器集成到微流体芯片中，以用于芯片实验室应用。

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