Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes

机译：使用组合锥形光纤生物传感器（CTFOB）浸入探针测量解离常数

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The document reports a novel method of measuring dissociation constant (k_D) of antibody-antigen interaction using evanescent wave based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring the dissociation constant of human Interleukin-6 (IL-6) and anti-IL-6 interaction. Sandwich immunoassay was used to generate fluorescence signal proportional to antigen-antibody conjugate pairs. A portable CCD based spectrometer setup was used to record spectral profile of the fluorescence signal. The measured value of dissociation constant k_D for IL-6 and capture anti-IL-6 (clone MQ2-13A5) antibodies at room temperature is 588± 9pM.

机译：该文献报道了一种新的方法，该方法使用基于fiber逝波的组合锥形光纤生物传感器（CTFOB）浸入探针来测量抗体-抗原相互作用的解离常数（k_D）。通过测量人白介素6（IL-6）的解离常数和抗IL-6相互作用证明了该方法。夹心免疫分析用于产生与抗原-抗体偶联物对成比例的荧光信号。基于便携式CCD的光谱仪设置用于记录荧光信号的光谱轮廓。 IL-6和捕获的抗IL-6（MQ2-13A5克隆）抗体在室温下的解离常数k_D的测量值为588±9pM。

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来源
《Optical fibers and sensors for medical diagnostics and treatment applications X》|2010年|P.75590A.1-75590A.4|共4页
会议地点 San Francisco CA(US)
作者
Chun-Wei Wang; Rakesh Kapoor;
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作者单位

Department of Physics, University of Alabama at Birmingham, Birmingham, AL 35294;

Department of Physics, University of Alabama at Birmingham, Birmingham, AL 35294;

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会议组织
原文格式 PDF
正文语种 eng
中图分类医用物理学;
关键词
cytokine; IL-6; fiber-optic; biosensor; fluorescence; dissociation constant; combination tapered.;

机译：细胞因子IL-6；光纤生物传感器荧光解离常数组合锥形。;

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专利

1. Errata: Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples [J] . Chun Wei Wang Upender Manne Vishnu B. Reddy Denise K. Oelschlager Venkat R. Katkoori William E. Grizzle and Rakesh Kapoor journal of biomedical optics . 2011,第1期

机译：勘误：用于定量评估血清样品中白介素-6的组合锥形光纤生物传感器浸入式探针的开发
2. Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples [J] . Chun Wei Wang, Upender Manne, Vishnu B. Reddy, Journal of biomedical optics . 2010,第6期

机译：用于定量评估血清样品中白介素6的组合锥形光纤生物传感器浸入式探针的开发
3. Measurements and analysis of dissociation rate constants of metal-fulvic acid complexes in aqueous solutions Part II: Measurement of decay rates by inductively-coupled plasma mass spectrometry and determination of rate constants for dissociation [J] . Sekaly ALR., Chakrabarti CL., Gregoire DC., Spectrochimica Acta, Part B. Atomic Spectroscopy . 1998,第6a8期

机译：水溶液中金属-富叶酸络合物的解离速率常数的测量和分析第二部分：通过电感耦合等离子体质谱法测定衰变速率并确定解离速率常数
4. Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes [C] . Chun-Wei Wang, Rakesh Kapoor SPIE Conference on Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications . 2010

机译：使用组合锥形光纤生物传感器（CTFOB）倾向探测的解离常数测量
5. Measurement of dissociation constants of some L-histidine derivatives and the formation constants of their complexes with methylmercury by NMR methods [D] . Yee, Diane C 1987

机译：NMR法测定某些L-组氨酸衍生物的解离常数及其与甲基汞的配合物的形成常数
6. Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes [O] . Chun-Wei Wang, Rakesh Kapoor -1

机译：使用组合锥形光纤生物传感器（CTFOB）倾向探测的解离常数测量
7. Errata: Development of combination tapered fiber-optic biosensor dip probe for quantitative estimation of interleukin-6 in serum samples [O] . Wang, Chun Wei, Manne, Upender, Reddy, Vishnu B., 2011

机译：勘误：用于定量评估血清样品中白介素-6的组合锥形光纤生物传感器浸入式探针的开发

Dissociation constant measurement using combination tapered fiber-optic biosensor (CTFOB) dip-probes

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