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Immunoassay demonstration using a local evanescent array coupled biosensor

机译:使用局部消逝阵列耦合生物传感器的免疫测定演示

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A Label-free optical waveguide immunosensor is investigated both theoretically and experimentally. The local evanescent array coupled (LEAC) biosensor is based on a local evanescent field shift mechanism, which differs from those of other evanescent waveguide sensors. Antigens specifically bound by immobilized antibodies on the waveguide surface increase the refractive index of the upper cladding of the waveguide, and hence shift the evanescent field distribution up. This local detection mechanism grants the LEAC sensor multi-analyte ability in a single optical path. Compared to traditional biosensors, including surface plasmon resonance and ring resonance biosensors, the non-resonant and temperature/wavelength insensitive properties of the LEAC biosensor relax its requirement on the optical source. It requires no accessory off-chip instruments such as spectrometers, making it a chip-scale biosensing platform. The on-chip detection is accomplished by integrating buried polysilicon detector arrays into silicon nitride waveguide in a commercial complementary metal oxide semiconductor (CMOS) process. Protein antigens and IgG producing biologically relevant antibody-antigen interactions were used to test the clinical utility of the LEAC biosensor platform. Advanced analysis beam propagation method (BPM) simulations and chips with different geometric parameters were used to study the relationship between the sensitivity and structure of LEAC biosensor.
机译:在理论和实验上都研究了无标签光波导免疫传感器。局部渐逝阵列耦合(LEAC)生物传感器基于局部渐逝场移位机制,该机制不同于其他渐逝波导传感器。通过固定在波导表面上的抗体特异性结合的抗原会增加波导上包层的折射率,从而使渐逝场分布向上移动。这种局部检测机制使LEAC传感器在单个光路上具有多种分析能力。与传统的生物传感器(包括表面等离子体共振和环形共振生物传感器)相比,LEAC生物传感器的非共振和温度/波长不敏感特性放宽了对光源的要求。它不需要诸如光谱仪之类的辅助芯片外仪器,使其成为芯片级生物传感平台。通过在商业互补金属氧化物半导体(CMOS)工艺中将掩埋的多晶硅检测器阵列集成到氮化硅波导中来完成片上检测。蛋白抗原和IgG产生生物学上相关的抗体-抗原相互作用被用来测试LEAC生物传感器平台的临床实用性。利用先进的分析光束传播方法(BPM)模拟和具有不同几何参数的芯片来研究LEAC生物传感器的灵敏度与结构之间的关系。

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