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首页> 外文会议>Optical Fibre Sensors pt.1 >Optical fibre biosensors for oxygen and glucose monitoring
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Optical fibre biosensors for oxygen and glucose monitoring

机译：用于氧气和葡萄糖监测的光纤生物传感器

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摘要

An optical fibre biosensor that uses an oxygen sensitive coating; Ruthenium complex [Dichlorotris(1,10-phenantroline)-rathenium(Ⅱ) hydrate], incorporated into an adhesive inorganic-organic hybrid polymer coating (ORMOCER~®) is described. The Ruthenium/ ORMOCER~® layer is used with optical fibres to form an extrinsic or intrinsic sensor. It can be applied to a microscope slide or lens to be interrogated by optical fibres, or form a cladding layer for an evanescent field optical fibre sensor. The Ruthenium complex is caused to fluoresce by a high brightness blue LED at 470 nm and the excitation light at 600nm is detected by a photomultiplier tube used as a photon counter, to measure fluorescence lifetime. The fluorescence is quenched by oxygen depletion within the layer, which can be linked to glucose by incorporation of suitable enzymes. The detection threshold is 0.7mg(O_2)/litre, and the sensitivity is 70 ns/mg per litre.
机译：一种使用氧气敏感涂层的光纤生物传感器；描述了掺入粘合剂-无机-有机杂化聚合物涂料（ORMOCER®）中的钌配合物[二氯三（1,10-菲咯啉）-钌（Ⅱ）水合物]。钌/ORMOCER®层与光纤一起使用以形成外在或本征传感器。它可以应用于要被光纤询问的显微镜载玻片或透镜，或形成用于瞬逝场光纤传感器的覆层。钌络合物通过470 nm的高亮度蓝色LED发出荧光，并通过用作光子计数器的光电倍增管检测600 nm的激发光，以测量荧光寿命。荧光通过该层内的氧消耗而淬灭，可以通过掺入合适的酶将其与葡萄糖连接。检测阈值为0.7mg（O_2）/升，灵敏度为70 ns / mg /升。

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