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首页> 外文会议>Optical Interactions with Tissue and Cells XVII; Progress in Biomedical Optics and Imaging; vol.7, no.7 >Monitoring tissue formation and organization of engineered tendon by optical coherence tomography
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Monitoring tissue formation and organization of engineered tendon by optical coherence tomography

机译：通过光学相干断层扫描技术监测工程肌腱的组织形成和组织

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摘要

The uniaxial orientation and bundle formation of collagen fibres determine the mechanical properties of tendons. Thus the particular challenge of tendon tissue engineering is to build the tissue with a highly organized structure of collagen fibres. Ultimately the engineered construct will be used as autologous grafts in tendon surgery, withstanding physiological loading. We grew pig tenocytes in porous chitosan scaffolds with multiple microchannels of 250-500 μm. The cell proliferation and production of extra-cellular matrix (ECM) within the scaffolds have been successfully monitored by Optical Coherence Tomography (OCT), a bench-top OCT system equipped with a broadband light source centred at 1300 nm. Under sterile condition, the measurements were performed on-line and in a non-destructive manner. In addition, a novel method based on OCT imaging, which calculates the occupation ratio of the microchannel derived from the scattered intensity has been developed. It is confirmed that the occupation ratio is correlated to cell proliferation and ECM production in the scaffolds. Thus this method has been utilised to assess the effect of different culture conditions on the tissue formation. The use of a perfusion bioreactor has resulted in a significantly (p < 1e~(-3)) higher cell proliferation and matrix production.
机译：胶原纤维的单轴取向和束形成决定了肌腱的机械性能。因此，肌腱组织工程的特殊挑战是用胶原纤维的高度组织化的结构来构建组织。最终，经过工程改造的构建体将在承受生理负荷的情况下用作肌腱手术中的自体移植物。我们在具有250-500μm多个微通道的多孔壳聚糖支架中生长猪肌腱细胞。支架内的细胞增殖和细胞外基质（ECM）的产生已通过光学相干断层扫描（OCT）成功监测，OCT是一种台式OCT系统，配备了以1300 nm为中心的宽带光源。在无菌条件下，测量以在线方式和非破坏性方式进行。此外，还开发了一种基于OCT成像的新方法，该方法可根据散射强度计算微通道的占有率。可以确定的是，占有率与支架中的细胞增殖和ECM产生相关。因此，该方法已用于评估不同培养条件对组织形成的影响。灌注生物反应器的使用已导致细胞增殖和基质产生显着（p <1e〜（-3））。

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