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Photobiostimulation on chondrocytes proliferation in different concentration of fetal bovine serum under low-level laser irradiation

机译:低剂量激光照射对不同浓度胎牛血清软骨细胞增殖的光生物刺激作用

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摘要

The aim of this in vitro study was to evaluate the influence of low-level laser irradiation (LLLI) on the chondrocytes proliferation cultured in different concentration of fetal bovine serum (FBS) using 658 nm, 785 nm and 830 nm diode lasers. The role of energy density (10-70 mJ·cm~(-2)) on chondrocytes proliferation following irradiation with 658 nm laser for 2 days was firstly investigated to find out the best laser energy density. Then the effect of LLLI on the proliferation of chondrocytes cultured with fetal bovine serum at 0%, 2%, 5% and 10% was also evaluated. The results showed that there was no or little photobiostimulation on the proliferation of chondrocytes cultured with 0% FBS and 10% FBS; the cell proliferation at 2% and 5% FBS was significantly modulated by LLLI.
机译:这项体外研究的目的是评估低水平激光照射(LLLI)对使用658 nm,785 nm和830 nm二极管激光器在不同浓度的胎牛血清(FBS)中培养的软骨细胞增殖的影响。首先研究了能量密度(10-70 mJ·cm〜(-2))对658 nm激光照射2天后软骨细胞增殖的作用,以找出最佳的激光能量密度。然后还评估了LLLI对用0%,2%,5%和10%胎牛血清培养的软骨细胞增殖的影响。结果表明,0%FBS和10%FBS培养的软骨细胞没有或几乎没有光生物刺激作用。 LLLI显着调节了2%和5%FBS下的细胞增殖。

著录项

  • 来源
    《Optical interactions with tissue and cells XXVI》|2015年|932111.1-932111.6|共6页
  • 会议地点 San Francisco CA(US)
  • 作者单位

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China,Fujian Key Laboratory of Developmental and Neural Biology, College of Life Sciences, Fujian Normal University, Fuzhou, 350108, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China;

    Fujian Key Laboratory of Developmental and Neural Biology, College of Life Sciences, Fujian Normal University, Fuzhou, 350108, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    photobiostimulation; chondrocyte; proliferation; fetal bovine serum;

    机译:光生物刺激;软骨细胞增殖;胎牛血清;

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