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首页> 外文会议>Optical interactions with tissue and cells XXVI >Time response of electrical conduction block in novel cardiomyocyte wire by extra-cellular photosensitization reaction at various irradiances
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Time response of electrical conduction block in novel cardiomyocyte wire by extra-cellular photosensitization reaction at various irradiances

机译：不同照射下细胞外光敏反应对新型心肌细胞电传导阻滞的时间响应

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摘要

We studied time response of electrical conduction (EC) block in a novel cardiomyocyte wire by extra-cellular photosensitization reaction (EPR) at various irradiances. This EC block using the EPR has been studied to develop a non-thermal arrhythmia therapeutic methodology. Despite the EC block in acute phase is needed to judge therapeutic endpoint in clinical arrhythmia therapy, time response of the EC block by the EPR in acute phase hasn't been studied. We measured the time to EC block occurrence by the EPR with intra-cellular Ca~(2+) concentration change using Fluo-4 AM fluorescence measurement by a confocal laser microscope system. The pattern cultivation cover glass with 10 mm Φ which had 60 μm width cultivation areas with 300 μm separations was used to form the cardiomyocyte wires. Rat cardiomyocyte with 10.8×10~5 cells was disseminated to the cover glasses installed in a 35 mm Φ dish. After 3 days from the dissemination, the EPR was operated to the cardiomyocyte wires for 10 min varying 3-120 mW/cm~2 in 663 nm laser irradiances with 20 μg/ml talaporfin sodium. An irradiation area was approximately 60 × 340 μm~2 on each wires. Cross correlation functions (CCF) in measured fluorescence images in every 10 s were calculated across the irradiation area. The time to EC block occurrence was defined as the time of the max difference between adjacent CCFs. By decreasing irradiances in 30-6 mW/cm~2, the time to EC block occurrence became longer from 294 to 434 s. In 30-120 mW/cm~2, the time to EC block occurrence was nearly constant in 300 s.
机译：我们通过在各种辐照下的细胞外光敏反应（EPR）研究了新型心肌细胞导线中电传导（EC）阻滞的时间响应。已经研究了使用EPR的EC阻滞，以开发一种非热性心律不齐的治疗方法。尽管在临床心律不齐治疗中判断急性期需要EC阻滞来判断治疗终点，但尚未研究EPR在急性期对EC阻滞的时间响应。我们使用共聚焦激光显微镜系统通过Fluo-4 AM荧光测量，通过EPR随细胞内Ca〜（2+）浓度变化测量了发生EC阻滞的时间。使用具有10mmΦ的图案培养盖玻片（其具有60μm宽度的培养区域且具有300μm的间隔）来形成心肌线。将具有10.8×10〜5个细胞的大鼠心肌细胞分散到安装在35 mmΦ皿中的盖玻片上。散播3天后，在20 nm / ml他拉泊芬钠的663 nm激光照射下，对心肌细胞线进行EPR 10分钟，变化3-120 mW / cm〜2。每条线上的照射面积约为60×340μm〜2。在整个照射区域中，每10秒计算一次荧光图像中的互相关函数（CCF）。 EC阻塞发生的时间定义为相邻CCF之间的最大差值的时间。通过以30-6 mW / cm〜2降低辐照度，达到EC阻滞的时间从294 s延长到434 s。在30-120 mW / cm〜2的时间内，发生EC阻塞的时间在300 s内几乎恒定。

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