• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV; Progress in Biomedical Optics and Imaging; vol.6 no.4 >Differences of response of human bladder cells to Photodynamic therapy (PDT) with Hypericum Perforantum L extract and Photofrinreg;
【24h】

Differences of response of human bladder cells to Photodynamic therapy (PDT) with Hypericum Perforantum L extract and Photofrinreg;

机译：贯叶连翘提取物和Photofrin®对人膀胱细胞对光动力疗法（PDT）的反应差异

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Refractory carcinoma in situ and resistant multifocaL transitional cell carcinoma (TCC) of the human urinary bladder respond modestly to PHOTOFRIN® (PⅡ) PDT. Hypericum perforatum L., (St. John's wort /Epirus' Vasalmo, Greece), a medicinal plant used for many human ailments, is under investigation as a new photosensitizer. We have reported on the antiproliferative activity of the lipophilic extract of the Hypericum perforatum L. (HP) against cultured T-24, and NET-11 bladder cancer cells. We investigated response of the polar methanolic fraction (PMF) of the HP extract versus PHOTOFRIN® in photodynamic therapy (PDT) of human bladder cancer cells, RT-4 and T-24.The PMF was extracted from the dry herb with methanol, followed by liquid extraction with petroleum ether. RT-4/T-24, were plated (10~5 cells/well) and placed in the incubator (37℃, 5%CO) for 24 hours prior to addition of drugs. PII 2ug/ml, or PMF 60ug /ml was added and incubation continued. After 24 hours, the cells were treated with laser light (630nm) with 0,1,2,4 and 8 Joules. The cells were then washed and reincubated for another 24 hours. After this incubation cell survival was assessed by the MTT assay. PMF-PDT induced percent cell kill of 0%, 0%, 0%, 29% and 75%, in RT-4 cells (primary noninvasive urinary bladder TCC) versus 5%, 9%, 13%, 69% and 86% , in T-24 cells(metastatic TTC) at 0,1,2,4 and 8 Joules respectively. PⅡ-PDT induced cell kill of 0%, 0% ,0%,0% and 9 %, in RT-4 cells versus 0%,10%,0%,21% and 77%, in T-24 cells at 0,1,2,4 and 8 Joules respectively.RT-24 cells were relatively more resistant than T-24 cells to PMF and PⅡ-PDT. Understanding mechanisms of such differential responses might prove useful.
机译：人膀​​胱顽固性原位癌和耐药性多灶性移行细胞癌（TCC）对PHOTOFRIN®（PⅡ）PDT的反应中等。贯叶连翘（Hypericum perforatum L。）（希腊圣约翰草/ Epirus'Vasalmo，希腊）是一种用于许多人类疾病的药用植物，目前正在研究作为一种新型光敏剂。我们已经报道了贯叶连翘（Hypericum perforatum L.）（HP）的亲脂性提取物对培养的T-24和NET-11膀胱癌细胞的抗增殖活性。我们研究了HP提取物的极性甲醇分数（PMF）与PHOTOFRIN®在人膀胱癌细胞RT-4和T-24的光动力疗法（PDT）中的反应。用石油醚萃取。将RT-4 / T-24铺板（10〜5个细胞/孔），并在加入药物之前在培养箱（37℃，5％CO）中放置24小时。加入2ug / ml的PII或60ug / ml的PMF，并继续孵育。 24小时后，用0、1、2、4和8焦耳的激光（630nm）处理细胞。然后洗涤细胞，再孵育24小时。孵育后，通过MTT测定评估细胞存活。 PMF-PDT在RT-4细胞（原发性非侵入性膀胱TCC）中诱导的细胞杀伤百分比分别为0％，0％，0％，29％和75％，而5％，9％，13％，69％和86％分别在0、1、2、4和8焦耳的T-24细胞（转移性TTC）中。在RT-4细胞中，PⅡ-PDT诱导的细胞杀伤率为0％，0％，0％，0％和9％，而在0时，T-24细胞的PⅡ-PDT诱导的细胞杀伤率为0％，10％，0％，21％和77％。 RT-24细胞对PMF和PⅡ-PDT的抗性要比T-24细胞高。了解这种差异反应的机制可能会很有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号